MRI: Acquisition of a CytoViva system for highly interdisciplinary research and education in nanoscience and nanotechnology

MRI：收购 CytoViva 系统，用于纳米科学和纳米技术的高度跨学科研究和教育

• 批准号: 1726095
• 负责人: Ioana Pavel
• 金额: $ 28.93万
• 依托单位: Wright State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-10-01 至 2021-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1726095&HistoricalAwards=false
• 关键词: MRI; Acquisition; CytoViva; system; highly

This project involves acquisition of a CytoViva optical microscope instrument that will benefit over 10 faculty and 100 undergraduate and graduate students (including underrepresented groups) at three Midwestern institutions of higher education, namely Wright State University (WSU), Otterbein University, Ohio, and Ball State University (BSU), Indiana. The cutting-edge equipment will facilitate the optical imaging and spectral confirmation of a wide range of nanomaterials in live biological systems or composite materials. Thereby, its acquisition will strengthen the research of these scientists and will lead to new interdisciplinary collaborations that will offer fundamental insight into critical nano-biomedical and environmental problems. The exploitation of the CytoViva system as a teaching tool will contribute to the development of modern academic curricula and the next generation of instrumentalists. Furthermore, over 100 high school students will be annually engaged in hands-on nano-related activities (Exploring STEMM Program at WSU). The scientific results and pedagogic materials will be shared with the scientific community at large and general public through various venues. The proposed CytoViva system will consist of a high signal-to-noise-enhanced dark-field optical microscope and an integrated hyperspectral imaging system. It will be equipped with a proprietary Dual Fluorescence Module, which will facilitate the real time, simultaneous imaging of both fluorescently-labeled and non-labeled nanomaterials with high spectral. The CytoViva technique has the following unique capabilities: a) no sample preparation and no alteration in the targeted environment, b) improved time- and cost-efficiency, c) pixel-specific spectral data, and d) the capability of differentiating between similar nanostructures based on finite differences in their aggregation state, orientation, and functionalization. The breadth of interdisciplinary research projects impacted by the CytoViva system will include a) identifying the broad-spectrum antiviral mechanism of silver nanoparticles against deadly viruses, b) developing methodologies for studying the cellular trafficking and transformations of noble metal nanomaterials for biomedical applications, c) understanding the molecular basis of gene expression regulation involved in the antibiotic resistance of pathogenic bacteria using gold nanoparticles functionalized with aptamers, d) treating and detecting docetaxel resistant prostate cancer by targeted nanomedical formulation, and e) cytosolic mapping of digitalis Na/K-ATPase complex internalization as a potentially new cellular signaling mechanism.

该项目涉及收购CytoViva光学显微镜仪器，这将使三所中西部高等教育机构的10多名教师和100多名本科生和研究生（包括代表性不足的群体）受益，即赖特州立大学（WSU），俄亥俄州奥特拜因大学和印第安纳州鲍尔州立大学（BSU）。这些尖端设备将有助于对活体生物系统或复合材料中的各种纳米材料进行光学成像和光谱确认。因此，它的收购将加强这些科学家的研究，并将导致新的跨学科合作，这将提供关键的纳米生物医学和环境问题的基本见解。CytoViva系统作为教学工具的开发将有助于现代学术课程和下一代乐器演奏家的发展。此外，每年将有100多名高中生参与与纳米相关的实践活动（探索WSU的STEMM计划）。科学成果和教学材料将通过各种渠道与广大科学界和公众分享。拟议的CytoViva系统将包括一个高信噪比增强的暗场光学显微镜和一个集成的高光谱成像系统。它将配备一个专有的双荧光模块，这将有助于真实的时间，同时成像的荧光标记和非标记的纳米材料与高光谱。CytoViva技术具有以下独特的能力：a）无需样品制备，也不会改变目标环境，B）提高了时间和成本效率，c）像素特异性光谱数据，以及d）基于聚集状态、取向和功能化的有限差异区分相似纳米结构的能力。受CytoViva系统影响的跨学科研究项目的广度将包括：a）确定银纳米颗粒对抗致命病毒的广谱抗病毒机制，B）开发用于研究生物医学应用的贵金属纳米材料的细胞运输和转化的方法，c）、了解基因表达调控的分子基础，涉及致病菌的抗生素耐药性，使用金纳米粒子功能化，适体，d）通过靶向纳米医学制剂治疗和检测多西他赛耐药前列腺癌，以及e）洋地黄Na/K-ATP酶复合物内化作为潜在的新细胞信号传导机制的胞质图谱。

项目成果

Assessment of Silver-Nanoparticles-Induced Erythrocyte Cytotoxicity through Ion Transport Studies

通过离子传输研究评估银纳米颗粒诱导的红细胞毒性

• DOI: 10.33594/000000156
• 发表时间: 2019
• 期刊: Cellular Physiology and Biochemistry
• 作者: Adragna, Norma C;Alla, Praveen K;Pavel-Sizemore, Ioana E;Paluri, Arathi S;Yaklic, Jerome;Lauf, Peter K
• 通讯作者: Lauf, Peter K