MRI: The Acquisition of Seahorse to measure Mitochondrial Metabolism will fuel Biological Research at an HBCU

MRI：获取海马来测量线粒体代谢将推动 HBCU 的生物学研究

• 批准号: 1726604
• 负责人: Michael Gitcho
• 金额: $ 26.26万
• 依托单位: Delaware State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-09-01 至 2020-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1726604&HistoricalAwards=false
• 关键词: MRI; Acquisition; Seahorse; measure; Mitochondrial

An award is made to Delaware State University to enhance research in several areas while also contributing to undergraduate education and training. The instrumentation (Seahorse, Argilent Technologies, Inc.) allows researchers to measure the energy and metabolism of the cell. In addition to providing training of undergraduates in research, this equipment will also be a critical component of the redesigned core curriculum in biology. The PIs of this project will pilot a course-based undergraduate research experience (CURE) that will provide an opportunity for students to participate in authentic research. Exposing students to such research experiences will give them a more positive view of scientific careers and enhance retention rates in STEM fields. This instrumentation will support a diverse, interdisciplinary team of investigators whose research focuses on understanding the genetic, molecular and cellular mechanisms that underlie energy budgets in cells (Seahorse). The Seahorse will serve four distinct projects. One project investigates the connection between mitochondrial function and TDP-43, a nuclear ribonucleoprotein that has been implicated in many forms of neurodegeneration. Two other projects investigate the role of dopamine in metabolic dysfunction related to neurodegeneration, one focusing on rotenone toxicity in a drosophila model, while the other project investigates the role of SUMOylation in preventing mitochondrial dysfunction and cell death. A fourth project investigates the role of CD44, a cell-surface glycoprotein important in the metabolic reprogramming of cells to glycolysis under hypoxic conditions.

特拉华州立大学获得了一个奖项，以加强在几个领域的研究，同时也为本科教育和培训做出贡献。该仪器（Seahorse, Argilent Technologies, Inc.）允许研究人员测量细胞的能量和新陈代谢。除了为本科生提供研究培训外，该设备还将成为重新设计的生物学核心课程的重要组成部分。该项目的pi将试行基于课程的本科研究体验（CURE），为学生提供参与真实研究的机会。让学生接触这样的研究经历将使他们对科学事业有更积极的看法，并提高STEM领域的保留率。该仪器将支持一个多样化的、跨学科的研究团队，他们的研究重点是了解细胞中能量预算的遗传、分子和细胞机制。海马将服务于四个不同的项目。其中一个项目研究了线粒体功能和TDP-43之间的联系，TDP-43是一种核糖核蛋白，与多种形式的神经变性有关。另外两个项目研究多巴胺在与神经变性相关的代谢功能障碍中的作用，一个项目关注果蝇模型中的鱼藤酮毒性，而另一个项目研究SUMOylation在防止线粒体功能障碍和细胞死亡中的作用。第四个项目研究CD44的作用，这是一种细胞表面糖蛋白，在缺氧条件下细胞代谢重编程到糖酵解中很重要。