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Developmental Architecture of Structure and Color on Butterfly Wings

蝴蝶翅膀结构和颜色的发育架构

基本信息

批准号：
1755329
负责人：
Brian Counterman
金额：
$ 72.2万
依托单位：
Mississippi State University
依托单位国家：
美国
项目类别：
Continuing Grant
财政年份：
2018
资助国家：
美国
起止时间：
2018-09-15 至 2021-01-31
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=1755329&HistoricalAwards=false
关键词：
Developmental Architecture Structure Color Butterfly

项目摘要

Color patterns are one of nature's most dynamically rich and important signals of communication among plants and animals. In nature, these colors are produced by either pigments or structures that reflect different wavelengths of light. This project leverages the dramatic diversity in butterfly wing color patterns to study the biological basis of pattern development. The relative simplicity and the accessibility of butterfly wings make them attractive model systems for the study of pattern formation. Using a combination of genome sequencing, microscopy and gene-editing, the project will identify the genes involved in the formation of complex color patterns. These results will provide valuable insights into basic biological processes that control cell and tissue development. The research will provide hands-on training for undergraduate and graduate students in molecular and computational biology methods. Through "Butterfly Roadshows" and other outreach events, the researchers will engage K-6 students with immersive activities that explore butterfly diversity using origami paper microscopes, which students can continue to use in their own backyards. Collectively, the outcomes of this research program will represent a major milestone for understanding the genetic and developmental processes that orchestrate color patterning and how complex, biologically-important color-based signals are generated in nature.The aim of this project is to explore the developmental genetics of structural coloration using butterfly wing patterns as a model system. Butterfly wings form large arrays of differentiating epithelial cells that determine scale fate, and ultimately, the final color pattern of the wing. Colors are the result of pigments and/or structures of individual wing scales that reflect different wavelengths of light. Unlike pigment-based colors, structural colors result from three-dimensional nanostructures that manipulate the behavior of light. Although the developmental and genetic basis of pigment-based variation is well understood in a wide array of organisms, little is known about the generative processes responsible for structural coloration. The natural arrangements of butterfly scale types in space, their sexual dimorphism, and the ability to assay position specific gene expression patterns across the wing throughout development provides an unparalleled opportunity to identify the genes and developmental processes responsible for patterning and activating highly specialized cellular states. Through a combination of transcriptome sequencing (RNA-seq), in situ hybridizations, immunofluorescence, electroporation and CRISPR genome editing, this study aspires to provide a direct connection between changes in gene networks and cytostructural variation. The project team members will also implement a multifaceted outreach and educational program that will (1) establish a vibrant Student Training Program, (2) actively recruit students from underrepresented groups, and (3) develop a public outreach program that engages students in hands-on, inquiry-based discovery. These efforts by the project team will ensure the impacts extend well beyond the specific research objectives.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

颜色模式是自然界中最具动态丰富性和最重要的动植物交流信号之一。在自然界中，这些颜色是由颜料或反射不同波长光的结构产生的。这个项目利用蝴蝶翅膀颜色图案的巨大多样性来研究图案发展的生物学基础。蝴蝶翅膀的相对简单性和可访问性使它们成为研究图案形成的有吸引力的模型系统。利用基因组测序，显微镜和基因编辑的组合，该项目将确定参与形成复杂颜色模式的基因。这些结果将为控制细胞和组织发育的基本生物学过程提供有价值的见解。这项研究将为本科生和研究生提供分子和计算生物学方法的实践培训。通过“蝴蝶路演”和其他推广活动，研究人员将让K-6学生参与沉浸式活动，使用折纸显微镜探索蝴蝶的多样性，学生可以继续在自己的后院使用。总的来说，这项研究计划的成果将代表一个重要的里程碑，了解遗传和发育过程，编排颜色图案，以及如何复杂的，生物重要的颜色为基础的信号是在自然界中产生的。该项目的目的是探索发育遗传学的结构着色使用蝴蝶翅膀图案作为模型系统。蝴蝶翅膀形成大量分化的上皮细胞，决定鳞片的命运，并最终决定翅膀的最终颜色图案。颜色是由个体翅膀鳞片的色素和/或结构反射不同波长的光的结果。与基于颜料的颜色不同，结构色是由操纵光行为的三维纳米结构产生的。虽然色素变异的发育和遗传基础在许多生物体中已被很好地理解，但对结构着色的生成过程知之甚少。蝴蝶鳞片类型在空间中的自然排列，它们的性别二态性，以及在整个发育过程中分析整个翅膀位置特异性基因表达模式的能力，为识别负责模式化和激活高度专业化细胞状态的基因和发育过程提供了无与伦比的机会。通过转录组测序（RNA-seq），原位杂交，免疫荧光，电穿孔和CRISPR基因组编辑的组合，这项研究旨在提供基因网络变化和细胞结构变异之间的直接联系。项目团队成员还将实施一个多方面的推广和教育计划，该计划将（1）建立一个充满活力的学生培训计划，（2）积极招募代表性不足的群体的学生，以及（3）制定一个公共推广计划，让学生参与实践，基于调查的发现。该奖项反映了NSF的法定使命，并通过使用基金会的知识价值和更广泛的影响审查标准进行评估，被认为值得支持。

项目成果

期刊论文数量（14）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Bringing immersive science to undergraduate laboratory courses using CRISPR gene knockouts in frogs and butterflies

DOI：
10.1242/jeb.208793
发表时间：
2020-02
期刊：
Journal of Experimental Biology
影响因子：
2.8
作者：
Arnaud Martin;Nora S. Wolcott;L. O’Connell
通讯作者：
Arnaud Martin;Nora S. Wolcott;L. O’Connell

Genome Assembly of the Dogface Butterfly Zerene cesonia

DOI：
10.1093/gbe/evz254
发表时间：
2019-11
期刊：
Genome Biology and Evolution
影响因子：
3.3
作者：
Luis Rodriguez-Caro;J. Fenner;Caleb Benson;Steven M. Van Belleghem;B. Counterman
通讯作者：
Luis Rodriguez-Caro;J. Fenner;Caleb Benson;Steven M. Van Belleghem;B. Counterman

Knockdowns of red Malphigian tubules reveal pigmentation roles in the milkweed bug

DOI：
10.1002/jez.b.23123
发表时间：
2022-02-21
期刊：
JOURNAL OF EXPERIMENTAL ZOOLOGY PART B-MOLECULAR AND DEVELOPMENTAL EVOLUTION
影响因子：
2.2
作者：
Francescutti, Caroline M.;Martin, Arnaud;Hanly, Joseph J.
通讯作者：
Hanly, Joseph J.

Impact of Artificial Diet on Development and Coloration in Zerene Cesonia

人工饲料对 Zerene Cesonia 发育和着色的影响