Research Initiation Award: Using Zebrafish (Danio rerio) as a model to assess the role of insulin receptor signaling in renal epithelial transport function.

研究启动奖：使用斑马鱼（Danio rerio）作为模型来评估胰岛素受体信号在肾上皮转运功能中的作用。

• 批准号: 1800781
• 负责人: Tracy Bell
• 金额: $ 30万
• 依托单位: University of Maryland Eastern Shore
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-04-01 至 2023-03-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1800781&HistoricalAwards=false
• 关键词: Research; Initiation; Award; Using; Zebrafish

Research Initiation Awards provide support for junior and mid-career faculty at Historically Black Colleges and Universities who are building new research programs or redirecting and rebuilding existing programs. It is expected that the award helps to further the faculty member's research capability and effectiveness, improves research and teaching at the home institution, and involves undergraduate students in research experiences. The award to the University of Maryland Eastern Shore will develop a novel methodology to assess the water and sodium regulation in vertebrates and will advance our knowledge of basic mechanism of vertebrate kidney transport and fluid balance.The goal of the project is to investigate the role of insulin in regulating sodium and water transport in the kidney. Epithelial cells that line the renal proximal tubule (PT)reabsorb a significant fraction of filtered sodium and water, and therefore, play a key role in regulating body fluid osmotic homeostasis. The sodium proton exchanger 3 (NHE3) is expressed in the PT and accounts for the majority of total Na+ and water epithelial transport. Insulin has been shown to stimulate Na+ and water epithelial transport in this segment via increased NHE3 activity. Zebrafish (Danio rerio) will be used as a model to further elucidate the signaling mechanisms that link insulin to an increase in proximal tubule specific sodium hydrogen proton exchanger 3 (NHE3) activity.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

研究启动奖为历史上黑人学院和大学的初级和中级职业教师提供支持，他们正在建立新的研究项目或重新定向和重建现有项目。预计该奖项将有助于进一步提高教师的研究能力和效率，改善家庭机构的研究和教学，并使本科生参与研究经验。马里兰州大学东海岸分校的获奖项目将开发一种新的方法来评估脊椎动物的水和钠调节，并将推进我们对脊椎动物肾脏转运和液体平衡基本机制的认识。该项目的目标是研究胰岛素在调节肾脏钠和水转运中的作用。排列在肾近端小管（PT）上的上皮细胞重吸收过滤的钠和水的显著部分，并且因此在调节体液渗透稳态中起关键作用。钠质子交换器3（NHE 3）在PT中表达，占总Na+和水上皮转运的大部分。胰岛素已被证明通过增加NHE3活性刺激该节段中的Na+和水上皮转运。斑马鱼（Danio rerio）将被用作进一步阐明胰岛素与近端小管特异性钠氢质子交换剂3（NHE 3）活性增加之间联系的信号机制的模型。该奖项反映了NSF的法定使命，并被认为值得通过使用基金会的智力价值和更广泛的影响审查标准进行评估来支持。