RUI: Collaborative Research: Understanding the role of a modified phosphotransferase system and a unique two component signal transduction system in regulating gene expression.
RUI:合作研究:了解修饰的磷酸转移酶系统和独特的两部分信号转导系统在调节基因表达中的作用。
基本信息
- 批准号:1817793
- 负责人:
- 金额:$ 30.8万
- 依托单位:
- 依托单位国家:美国
- 项目类别:Standard Grant
- 财政年份:2018
- 资助国家:美国
- 起止时间:2018-08-01 至 2024-07-31
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Bacteria have the ability to directly sense their environment and change their behavior according to their surroundings. While the behavioral changes in the bacteria can be seen visually, the intricate genetic processes that lead to some of the changes are not fully understood. This project will investigate some of the ways in which bacteria carry out these processes using Sinorhizobium meliloti as a model. Sinorhizobium meliloti is a member of a larger group of bacteria called rhizobia, and is environmentally and economically vital in the field of agriculture. Sinorhizobium beneficially infects legumes and provides useable nitrogen to increase crop yield. An efficient and productive rhizobium infection reduces the need for chemical fertilizers. Expanding our knowledge of the genetic systems that control the behavior of this bacterium has the potential to allow manipulation to optimize the rhizobium-plant interaction. The project will train students from the across educational levels, from high school to graduate students to do scientific work in this field and to communicate it properly. The overarching goal of this proposal is to investigate and understand the role of a hybrid phosphotransferase system (PTS) and a unique two component signal transduction system in regulating gene expression in the bacterial nitrogen-fixing gram-negative symbiont Sinorhizobium meliloti. The project will study a recently identified and novel interaction between the two systems that regulates several cellular processes. Additionally, the systems themselves are interesting and insufficiently characterized: the phosphotransferase system is an abbreviated nitrogen-sugar hybrid while the two-component system has an intriguing multiple-sensing domain HWE kinase (Sma0113) and an atypical response regulator with no effector domain (Sma0114). One of the processes under dual regulation is catabolite repression. This is a complex process through which bacterial cells adapt to obtain energy and raw materials, and, in related bacteria, controls the expression of genes associated to their ability to interact with their hosts. Three approaches will be used to elucidate how the systems are integrated into the cellular network. Transcriptome analysis will allow identification of the combined regulon of the two systems. Genetic, biochemical, and proteomic methods will reveal their downstream partners. Genetic tools will be used to characterize the promoter structure that allows dual regulation. The results will bring better understanding of the characteristics of the two systems and the role each plays in regulating gene expression in S. meliloti and similar gram negative bacteria.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.
细菌有能力直接感知环境,并根据环境改变自己的行为。虽然细菌的行为变化可以用肉眼看到,但导致一些变化的复杂遗传过程并不完全清楚。这个项目将以苜蓿中华根瘤菌为模型,研究细菌进行这些过程的一些方式。苜蓿中华根瘤菌是根瘤菌中的一员,在农业领域具有重要的环境和经济价值。中华根瘤菌有益于感染豆类植物,并提供可利用的氮素,以增加作物产量。高效多产的根瘤菌感染减少了对化肥的需求。扩大我们对控制这种细菌行为的遗传系统的了解,有可能使操纵优化根瘤菌与植物的相互作用成为可能。该项目将培训不同教育水平的学生,从高中到研究生,从事这一领域的科学工作,并进行适当的沟通。这项建议的首要目标是调查和了解混合磷酸转移酶系统(PTS)和独特的两组分信号转导系统在细菌固氮革兰氏阴性共生菌Meliloti中调控基因表达的作用。该项目将研究最近发现的两个系统之间的一种新颖的相互作用,该相互作用调节着几个细胞过程。此外,这些系统本身很有趣,但还不够充分:磷酸转移酶系统是一个缩写的氮-糖混合体,而双组分系统具有一个有趣的多敏感结构域HWE激酶(Sma0113)和一个没有效应结构域的非典型反应调节因子(Sma0114)。双重调控下的一个过程是分解代谢抑制。这是一个复杂的过程,细菌细胞通过它来适应获得能量和原料,并在相关细菌中控制与其与宿主相互作用的能力相关的基因的表达。将使用三种方法来阐明这些系统如何集成到蜂窝网络中。转录组分析将有助于识别这两个系统的联合调控。遗传、生化和蛋白质组学方法将揭示它们的下游伙伴。基因工具将被用来描述允许双重调控的启动子结构。这一结果将使人们更好地了解这两个系统的特点以及每个系统在调节苜蓿沙门氏菌和类似的革兰氏阴性细菌中的基因表达方面所起的作用。这一奖项反映了NSF的法定使命,并通过使用基金会的智力优势和更广泛的影响审查标准进行评估,被认为值得支持。
项目成果
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