Impact of the Neuropeptide S system and NPS-receptor polymorphisms on fear and anxiety (A08)

A08项目的目的是研究人类相关NPSR1多态(N107I)的功能特征。一种类似的突变(I107N)被引入到一种新的小鼠模型中，该模型使用CRISPR/Cas9介导的基因编辑。这种突变对神经元特性、恐惧相关脑区(如杏仁核、丘脑内层)神经元回路、恐惧消退和泛化的功能后果将在体外/体外使用电生理学和光遗传学技术进行分析，并将在体内使用已建立的行为和应激范式进行分析。

Aim of the project A08 is the functional characterization of the human-relevant NPSR1 polymorphism (N107I). An analogous mutation (I107N) has been introduced into a new mouse model using CRISPR/Cas9-mediated gene editing. The functional consequence of this mutation on neuronal properties, on neuronal circuits in fear relevant brain regions (e.g. amygdala, intralaminar thalamus), fear extinction and generalization will be analyzed in vitro/ex vivo using electrophysiological and optogenetic techniques and in vivo using established behavioral- and stress-paradigms.