Complex study and physical mapping of genes in hexaploid wheat, responsible for embryo development of wheat-rye hybrids via interaction with rye genome

六倍体小麦基因的复杂研究和物理定位，通过与黑麦基因组的相互作用负责小麦-黑麦杂交种的胚胎发育

• 批准号: 237881595
• 负责人: Professor Dr. Andreas Börner
• 金额: --
• 依托单位: Abteilung Genbank
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2013
• 资助国家: 德国
• 起止时间: 2012-12-31 至 2019-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/237881595?language=en
• 关键词: Complex; study; physical; mapping; genes

One of the reproductive barriers that can isolate species is embryo lethality which is due to dysfunctional interaction between parental genomes. Embryo lethality obtained in crosses of hexaploid wheat with diploid rye is the result of complement interaction between the two genes/alleles Eml-A1 and Eml-R1b from wheat and rye, respectively. In addition, the 1D wheat chromosome carries unknown genetic factor(s) which have a strong effect on the viability of wheat-rye hybrid seeds. The aim of the previous work was the study and mapping of the loci in hexaploid wheat (Eml-A1, which is located on chromosome 6A and another unknown locus(loci) located on chromosome 1D), responsible for embryo and endosperm development of wheat-rye hybrids via interaction with the rye Genome.The goals of the project renewal are: (i) determination of the genetic identity of Eml-R1b and Eml-A1 loci in amphihaploid, amphidiploid and parental forms by GBS analysis; (ii) study of the nature of sterility in amphidiploid (ADL2) wheat-rye hybrids (iii) candidate gene selection and detailed analysis of gene expression in apical meristem of embryos in wheat-rye hybrids with Eml-A1 and Eml-R1b alleles by RNA-sequencing and (iv) identification of the function of Dee-D1 locus on wheat chromosome 1D. Further new information about genes involved in apical meristem development in early stages of embryogenesis of wheat-rye hybrids (and of other plants) will be gained.

能够隔离物种的生殖障碍之一是胚胎致死，这是由于亲本基因组之间的功能失调的相互作用。在六倍体小麦与二倍体黑麦的杂交中获得的胚胎致死性是分别来自小麦和黑麦的两个基因/等位基因Eml-A1和Eml-R1 b之间的互补相互作用的结果。此外，小麦一维染色体携带未知的遗传因子，这些遗传因子对小麦-黑麦杂种种子的生活力有很大的影响。前期工作的目的是研究和定位六倍体小麦中的基因座（Eml-A1，位于染色体6A上，另一个未知位点位于染色体1D上），通过与黑麦基因组的相互作用负责小麦-黑麦杂种的胚和胚乳发育。（i）通过GBS分析确定双单倍体、双二倍体和亲本形式中Eml-Rlb和Eml-A1基因座的遗传同一性;（ii）研究双二倍体（ADL 2）小麦-黑麦杂种的不育性的性质（iii）候选基因的选择和详细的基因表达的分析在小麦黑麦杂种与EML-A1和EML-R1 b等位基因的胚胎顶端分生组织的RNA测序和（iv）鉴定Dee的功能，小麦1D染色体上的D1位点。进一步的新的信息，基因参与小麦黑麦杂种（和其他植物）的胚胎发生早期阶段的顶端分生组织的发展将获得。