MRI: Acquisition of a wide-field inverted fluorescent microscope capable of live-cell tracking to support faculty research and undergraduate education at Stetson University

MRI：购买能够进行活细胞跟踪的宽视场倒置荧光显微镜，以支持斯泰森大学的教师研究和本科生教育

• 批准号: 1920029
• 负责人: Holley Lynch
• 金额: $ 26.61万
• 依托单位: Stetson University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-10-01 至 2023-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1920029&HistoricalAwards=false
• 关键词: MRI; Acquisition; wide; field; inverted

An award is made to Stetson University, a predominantly undergraduate university in Central Florida, to purchase an inverted fluorescent microscope system capable of imaging over a wide range of living biological samples from subcellular structures to small organisms. This instrumentation will actively engage undergraduate students across Biology, Health Sciences, and Physics in superior training through faculty-mentored research projects and promote implementation of inquiry-based lab experiences for students in upper-level Biology and Physics courses, thus providing exposure to advanced microscopy for a diverse population of students. The microscope system will propel cutting-edge research programs at Stetson University and foster sophisticated capstone research for seniors under the mentorship of faculty members committed to student training and advancing the participation of under- represented and minority undergraduates in the sciences. Student outcomes include opportunities to present their work at regional and national scientific conferences and as coauthors on scientific papers. Collectively, these invaluable experiences will promote scientific competency in students as they achieve a comprehensive range of career goals and contribute to the development of the next generation of scientists. Furthermore, the acquisition of this microscope system will provide a regional resource for other researchers in Central Florida, as well as enable exciting cellular-based science outreach programs through art exhibits based on images acquired using this microscope and expand Stetson?s existing relationships with local K- 12 schools through demonstrations and hands-on activities using this instrumentation to further promote scientific literacy.This microscope will provide a powerful foundation to support and enable fundamental pioneering research by junior faculty members. Highlights of the research that will be enabled through this imaging system include: 1) tracking migrating cells in living tissues to link cellular and subcellular mechanisms to tissue-scale movements; 2) examining improperly located cell death proteins in malignant cells; 3) dissecting communication pathways that regulate cardiac myocyte cell proliferation and regeneration in Ciona intestinalis; 4) examining spatial organization and dynamics of iridescent marine bacteria; and 5) promoting education through use of CRISPR/Cas9 gene editing technology by undergraduates in a Genetics course to manipulate and label genes in order to visualize effects in living organisms. These diverse interdisciplinary projects, made possible through the acquisition of this microscope, represent exciting educational opportunities for undergraduate students to contribute to meaningful scientific discoveries.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

佛罗里达州中部的斯泰森大学 (Stetson University) 获得了一项奖励，购买倒置荧光显微镜系统，该系统能够对从亚细胞结构到小型生物体的各种活体生物样本进行成像。该仪器将通过教师指导的研究项目，积极让生物学、健康科学和物理学的本科生接受高级培训，并促进高级生物学和物理学课程学生实施基于探究的实验室体验，从而为不同群体的学生提供先进显微镜的接触机会。该显微镜系统将推动斯泰森大学的尖端研究项目，并在致力于学生培训和促进代表性不足和少数族裔本科生参与科学的教职人员的指导下，促进高年级学生的复杂顶点研究。学生的成果包括有机会在地区和国家科学会议上展示他们的作品以及作为科学论文的合著者。总的来说，这些宝贵的经验将提高学生的科学能力，帮助他们实现全面的职业目标，并为下一代科学家的发展做出贡献。此外，购买该显微镜系统将为佛罗里达州中部的其他研究人员提供区域资源，并通过基于使用该显微镜获取的图像的艺术展览来实现令人兴奋的基于细胞的科学推广计划，并通过使用该仪器进行演示和实践活动扩大 Stetson 与当地 K-12 学校的现有关系，以进一步提高科学素养。该显微镜将为支持和实现初级开创性研究提供强大的基础。 教职人员。通过该成像系统将实现的研究重点包括：1）跟踪活组织中的迁移细胞，将细胞和亚细胞机制与组织尺度的运动联系起来； 2) 检查恶性细胞中位置不正确的细胞死亡蛋白； 3）剖析玻璃海鞘调节心肌细胞增殖和再生的通讯途径； 4）检查虹彩海洋细菌的空间组织和动态； 5) 本科生在遗传学课程中使用 CRISPR/Cas9 基因编辑技术来操纵和标记基因，以可视化对生物体的影响，从而促进教育。通过购买这款显微镜，这些多样化的跨学科项目成为可能，为本科生提供了令人兴奋的教育机会，为有意义的科学发现做出贡献。该奖项反映了 NSF 的法定使命，并通过使用基金会的智力价值和更广泛的影响审查标准进行评估，被认为值得支持。