RUI: SG: Collaborative Research: Phylogenomics and diversification of the snapping shrimp genus Alpheus

RUI：SG：合作研究：鳄虾属 Alpheus 的系统基因组学和多样化

• 批准号: 1924675
• 负责人: Kristin Hultgren
• 金额: $ 10.57万
• 依托单位: Seattle University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-09-01 至 2024-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1924675&HistoricalAwards=false
• 关键词: RUI; SG; Collaborative; Research; Phylogenomics

Snapping shrimp in the genus Alpheus, known for their spectacular color patterns and the explosive snap created by their major claw, are one of the most diverse and abundant animal groups in tropical coral reefs. Snapping shrimp form mutualistic relationships with a diversity of partners such as corals and fish and are emerging as a model group for understanding symbiosis and species diversification in marine environments. However, such studies require a more complete understanding of how many different species of Alpheus exist worldwide, and the relationships among these species. This research will address these questions by building an evolutionary tree of the group with next-generation DNA sequencing data. These data will be used to test whether factors such as host symbioses or color patterns drove the spectacular diversification of this group. Studying the biodiversity of this group will help to understand more broadly the origins and maintenance of biodiversity in shallow marine tropical ecosystems. Species pages will be created through the Encyclopedia of Life for public outreach and educational purposes, and a partnership with a science museum will develop workshops in science communication for undergraduates. A graduate student, and multiple undergraduates will also be trained in genetic barcoding, species delimitation, species descriptions, and comparative evolutionary analyses. This research will produce the first comprehensive global phylogeny of snapping shrimp in the genus Alpheus, spanning 50% of the 300+ described species and using phylogenomic data generated via anchored hybrid enrichment. This phylogeny will be used to test long-standing taxonomic and morphological questions in Alpheus, such as the monophyly of morphologically defined species groups and the role of habitat use in driving instances of morphological convergence. A comprehensive genetic barcoding survey of field and museum specimens will help estimate the existing worldwide biodiversity of the group. Using phylogenetic reconstructions, fieldwork, and color pattern analyses, researchers will test whether color patterns - long hypothesized to have systematic importance - are important factors in establishing species boundaries. Finally, the phylogenomic tree and data on worldwide patterns of species diversity will be used to test whether species diversification in Alpheus is linked to ecological factors such as symbiosis.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

Alpheus属的对虾以其壮观的颜色图案和大爪子造成的爆炸性爆裂而闻名，是热带珊瑚礁中种类最多、数量最多的动物群体之一。捕虾与珊瑚和鱼类等各种伙伴形成了互惠互利的关系，并正在成为了解海洋环境中的共生和物种多样性的模范群体。然而，这样的研究需要更全面地了解世界上有多少不同的阿尔菲斯物种，以及这些物种之间的关系。这项研究将通过使用下一代DNA测序数据建立该群体的进化树来解决这些问题。这些数据将被用来测试寄主共生或颜色模式等因素是否推动了这一群体惊人的多样化。研究这一群体的生物多样性将有助于更广泛地了解浅海热带生态系统生物多样性的起源和维持。将通过生命百科全书创建物种页面，用于公共宣传和教育目的，并将与一家科学博物馆合作，为本科生举办科学交流讲习班。一名研究生和多名本科生还将接受遗传条形码、物种划分、物种描述和比较进化分析方面的培训。这项研究将利用锚定杂交富集法产生的系统发育数据，首次建立Alpheus属对虾的全面全球系统发育，涵盖所描述的300多个物种中的50%。这一系统发育将被用来测试Alpheus中长期存在的分类学和形态问题，例如形态定义的物种群体的单系性，以及栖息地利用在推动形态趋同实例中的作用。对野外和博物馆标本进行全面的遗传条形码调查将有助于估计该群体现有的全球生物多样性。通过系统发育重建、田野调查和颜色模式分析，研究人员将测试颜色模式--长期以来被认为具有系统重要性--是否是建立物种边界的重要因素。最后，系统基因组树和全球物种多样性模式的数据将被用来测试阿尔菲斯的物种多样性是否与共生等生态因素有关。这一奖项反映了NSF的法定使命，并通过使用基金会的智力优势和更广泛的影响审查标准进行评估，被认为值得支持。

项目成果

Correlated evolution of larval development, egg size and genome size across two genera of snapping shrimp

两个鳄虾属幼体发育、卵大小和基因组大小的相关进化

• DOI: 10.1111/jeb.13945
• 发表时间: 2021
• 期刊: Journal of Evolutionary Biology
• 影响因子: 2.1
• 作者: Hultgren, Kristin M.;Chak, Solomon T. C.;Bjelajac, Jeremy;Macdonald, Kenneth S.
• 通讯作者: Macdonald, Kenneth S.