Collaborative Research: RUI: Multi-mutualist Effects on Populations, Communities, and Ecosystems Across Ecological Gradients

合作研究：RUI：跨生态梯度对人口、社区和生态系统的多重互利效应

• 批准号: 1927671
• 负责人: Emily Grman
• 金额: $ 59.91万
• 依托单位: Eastern Michigan University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-09-01 至 2024-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1927671&HistoricalAwards=false
• 关键词: Collaborative; Research; RUI; Multi; mutualist

Restoration, or the rebuilding of degraded ecosystems, may be one of the best tools for slowing the loss of biodiversity. Unfortunately, many rare plant species fail to establish in restored ecosystems, potentially because the soil microorganisms required for their establishment are missing. Most plant species interact with specific soil microbes, but the conditions under which those microbes (microscopic organisms) benefit or inhibit the establishment of rare plants is unknown, limiting our ability to increase the success of ecosystem restoration. This work will test the overarching hypothesis that the presence of particularly rare, difficult-to-establish prairie legumes is limited by both mycorrhizal and rhizobial mutualists and that in low-fertility environments those mutualists synergistically improve prairie restoration outcomes in terms of legume establishment, plant diversity, and ecosystem function. Further, it will evaluate whether these interactions matter in the real world. In this research, undergraduate and graduate student researchers will investigate two groups of microbes that live in the soil, known to enhance the growth of rare legumes (plants in the bean family) in native prairies. Researchers will isolate strains of these microbes from restored ecosystems and from relatively undisturbed native prairie ecosystems and then evaluate their ability (either singly or in combination) to increase legume growth, increase prairie plant community diversity, and improve prairie ecosystem performance across a wide range of environmental conditions. They will also share these beneficial microorganisms with restoration practitioners and train practitioners to incorporate soil microbes in real-world, large-scale prairie restoration projects. With this award, researchers will examine mutualisms between legumes and soil rhizobial bacteria and arbuscular mycorrhizal fungi, using a range of approaches that span the spectrum of highly controlled lab studies to real-world field studies in restored prairies. In plant growth chambers and greenhouses, researchers will test the quality of individual strains of rhizobia and arbuscular mycorrhizal fungi isolated from various remnant and restored prairie soils and evaluate whether they can synergistically enhance the growth of twelve prairie legumes under unfavorable soil conditions, including decreased nutrient availability or poor microbial quality. In field plots across ten restored prairies varying in soil fertility, they will evaluate whether patterns from controlled environments remain consistent for legume growth in real prairies. In greenhouse mesocosms and field plots in restored prairies, researchers will evaluate whether both types of mutualists together have synergistically positive effects on plant community diversity and prairie ecosystem functions (primary production, nutrient cycling, soil building).This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

恢复或重建退化的生态系统可能是减缓生物多样性丧失的最佳工具之一。不幸的是，许多稀有植物物种未能在恢复的生态系统中建立，可能是因为它们建立所需的土壤微生物缺失。大多数植物物种与特定的土壤微生物相互作用，但这些微生物（微生物）有益或抑制稀有植物建立的条件尚不清楚，限制了我们提高生态系统恢复成功率的能力。这项工作将测试的总体假设，特别是罕见的，难以建立草原豆科植物的存在是有限的菌根和根瘤共生，在低生育力的环境中，这些共生协同提高草原恢复结果的豆科植物的建立，植物多样性和生态系统功能。此外，它还将评估这些相互作用在真实的世界中是否重要。在这项研究中，本科生和研究生研究人员将调查两组生活在土壤中的微生物，已知它们可以促进原生草原中稀有豆类（豆类植物）的生长。研究人员将从恢复的生态系统和相对未受干扰的原生草原生态系统中分离出这些微生物的菌株，然后评估它们（单独或组合）增加豆科植物生长的能力，增加草原植物群落多样性，并在广泛的环境条件下改善草原生态系统性能。他们还将与恢复从业者分享这些有益的微生物，并培训从业者将土壤微生物纳入现实世界的大规模草原恢复项目中。有了这个奖项，研究人员将研究豆科植物和土壤根瘤菌和丛枝菌根真菌之间的互惠关系，使用一系列方法，这些方法涵盖了高度控制的实验室研究到恢复草原的真实实地研究。在植物生长室和温室中，研究人员将测试从各种残留和恢复的草原土壤中分离出的根瘤菌和丛枝菌根真菌的单个菌株的质量，并评估它们是否可以在不利的土壤条件下协同促进12种草原豆科植物的生长，包括养分供应减少或微生物质量差。在10个土壤肥力不同的恢复草原的田间试验中，他们将评估受控环境的模式是否与真实的草原中的豆类生长保持一致。在温室中生态系统和恢复草原的田间小区中，研究人员将评估这两种类型的互利共生者是否共同对植物群落多样性和草原生态系统功能（初级生产，养分循环，土壤建设）产生协同积极影响。该奖项反映了NSF的法定使命，并通过使用基金会的知识价值和更广泛的影响审查标准进行评估，被认为值得支持。

项目成果

Inoculation with remnant prairie soils increased the growth of three native prairie legumes but not necessarily their associations with beneficial soil microbes

• DOI: 10.1111/rec.13126
• 发表时间: 2020-02-23
• 期刊: RESTORATION ECOLOGY
• 影响因子: 3.2
• 作者: Grman, Emily;Allen, Jamie;Price, Paul A.
• 通讯作者: Price, Paul A.