Chromatin regulation at promoters and enhancers, a molecular-mechanistic analysis (A02)

启动子和增强子的染色质调控，分子机制分析 (A02)

• 批准号: 241353021
• 金额: --
• 依托单位: Genzentrum - Laboratorium für molekulare Biologie
• 依托单位国家: 德国
• 项目类别: Collaborative Research Centres
• 财政年份: 2013
• 资助国家: 德国
• 起止时间: 2012-12-31 至 2018-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/241353021?language=en
• 关键词: Chromatin; regulation; promoters; enhancers; molecular

Our goal is to understand the regulation of gene expression in Drosophila development, and in particular the interplay between transcription factor activity and chromatin organization. Building on a comprehensive genome-wide analysis of core promoters, we plan to use sensitive proteomics approaches to characterize the interactome of selected general transcription machinery components and histone modifications in a promoter-type specific fashion. In a second aim, we seek to characterize the role of pioneer transcription factors in regulating chromatin accessibility at enhancers within the Ecdysone signaling cascade.

我们的目标是了解果蝇发育中基因表达的调控，特别是转录因子活性和染色质组织之间的相互作用。基于对核心启动子的全面全基因组分析，我们计划使用敏感的蛋白质组学方法来表征选定的通用转录机制组件和启动子类型特异性方式的组蛋白修饰的相互作用组。第二个目标是，我们试图表征先锋转录因子在调节蜕皮激素信号级联增强子染色质可及性中的作用。