Chromatin regulation at promoters and enhancers, a molecular-mechanistic analysis (A02)

我们的目标是了解果蝇发育中基因表达的调控，特别是转录因子活性和染色质组织之间的相互作用。建立在一个全面的全基因组分析的核心启动子，我们计划使用敏感的蛋白质组学方法来表征的相互作用组的选择一般的转录机制组件和组蛋白修饰的启动子类型的特定方式。在第二个目标中，我们试图表征先锋转录因子在调节蜕皮激素信号级联中增强子处染色质可及性中的作用。

Our goal is to understand the regulation of gene expression in Drosophila development, and in particular the interplay between transcription factor activity and chromatin organization. Building on a comprehensive genome-wide analysis of core promoters, we plan to use sensitive proteomics approaches to characterize the interactome of selected general transcription machinery components and histone modifications in a promoter-type specific fashion. In a second aim, we seek to characterize the role of pioneer transcription factors in regulating chromatin accessibility at enhancers within the Ecdysone signaling cascade.