Histone H4K20 demethylation in cell cycle-controlled differentiation (A12)

细胞周期控制分化中的组蛋白 H4K20 去甲基化 (A12)

• 批准号: 241375609
• 金额: --
• 依托单位: Lehrstuhl Molekularbiologie
• 依托单位国家: 德国
• 项目类别: Collaborative Research Centres
• 资助国家: 德国
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/241375609?language=en
• 关键词: Histone; H4K20; demethylation; cell; cycle

Our current results from the model organism Xenopus indicate that Histone H4K20me1 and -me2 levels are regulated by S-phase dilution in proliferating cells, while becoming adjusted by specific histone demethylases such as PHF8, RSBN1 and HR23B in postmitotic, differentiating cells. By combining gain and loss of function analysis with RNA-Seq and ChIP-Seq approaches in ectodermal organoid cultures, we plan to dissect, how important these enzymes are for balancing the embryonic H4K20 methylation landscape, and how this is crucial for the successful differentiation of epidermal cells during development.

我们目前的研究结果从模式生物爪蟾表明，组蛋白H4 K20 me 1和-me 2水平的调节增殖细胞中的S期稀释，而成为有丝分裂后，分化细胞中的特定组蛋白去甲基化酶，如PHF 8，RSBN 1和HR 23 B调整。通过在外胚层类器官培养物中将功能分析的获得和丧失与RNA-Seq和ChIP-Seq方法相结合，我们计划剖析这些酶对于平衡胚胎H4 K20甲基化景观的重要性，以及这对于发育过程中表皮细胞的成功分化至关重要。