Histone H4K20 demethylation in cell cycle-controlled differentiation (A12)

我们目前在模式生物非洲爪哇的研究结果表明，在增殖细胞中，组蛋白H4K20me1和-me2的水平受到S期稀释的调节，而在有丝分裂后、分化的细胞中，组蛋白H4K20me1和-me2的水平则受到特定的组蛋白去甲基酶的调节，如PHF8、RSBN1和HR23B。通过结合外胚层器官培养中的功能得失分析与RNA-Seq和ChIP-Seq方法，我们计划剖析这些酶对于平衡胚胎H4K20甲基化格局的重要性，以及这对表皮细胞在发育过程中的成功分化是如何至关重要的。

Our current results from the model organism Xenopus indicate that Histone H4K20me1 and -me2 levels are regulated by S-phase dilution in proliferating cells, while becoming adjusted by specific histone demethylases such as PHF8, RSBN1 and HR23B in postmitotic, differentiating cells. By combining gain and loss of function analysis with RNA-Seq and ChIP-Seq approaches in ectodermal organoid cultures, we plan to dissect, how important these enzymes are for balancing the embryonic H4K20 methylation landscape, and how this is crucial for the successful differentiation of epidermal cells during development.