MRI: Acquisition of a Seahorse XFe96 Analyzer for High-Throughput Measurements of Glycolysis and Mitochondrial Respiration at Skidmore College

MRI：斯基德莫尔学院购买 Seahorse XFe96 分析仪，用于糖酵解和线粒体呼吸的高通量测量

• 批准号: 2018952
• 负责人: Sarita Lagalwar
• 金额: $ 26.65万
• 依托单位: Skidmore College
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-08-01 至 2023-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2018952&HistoricalAwards=false
• 关键词: MRI; Acquisition; Seahorse; XFe96; Analyzer

An award is made to Skidmore College to support the purchase of instrumentation for measuring subtle, real-time changes in mitochondrial respiration by two primary output measurements: oxygen consumption and extracellular acidification. The 96-well automated format allows for high throughput analysis of small sample volumes with high accuracy and high resolution. The analyzer will be used by faculty and students in the Neuroscience Program and the Departments of Biology, Chemistry and Health & Human Physiological Sciences for basic science research, translational research and teaching. Experimental use and data analysis will be integrated into multiple courses across the undergraduate science curriculum, thereby reaching a broad array of undergraduate users. Furthermore, the instrument will be featured in summer workshops for Skidmore Scholars in Science and Math (S3M). S3M scholars are rising sophomores who are strong science students from underrepresented backgrounds in STEM. It is expected that exposure to the instrument and analytical practice through courses and the S3M workshop will lead students to incorporate the instrument into their student-driven independent research under the guidance of their faculty.The analyzer works with a wide variety of samples including cultured cells, excised tissue, bacteria and small animal embryos making it ideal for the various research interests of Skidmore faculty. Immediate research uses by the PI and co-PIs include investigation of mitochondrial function in neuronal cells, the impact of aging on muscle and endothelial cells, the identification and screening of novel respiratory genes in C. elegans mutants, identification of endocrine control of fish gill ionocyte metabolism, and investigation of metal homeostasis in bacteria. The acquisition of the instrument coincides with the opening of Skidmore College’s new Center for Integrated Sciences, a new science building which brings together all 10 STEM departments and programs under one roof. Importantly, the intellectual merit regarding the shared use of the instrument is in line with the College’s mission to integrate research and teaching within the sciences. Additionally, the instrument will contribute to existing active and future collaborations between Skidmore scientists and outside researchers. Data acquired on the new system will be disseminated in peer-reviewed publications and through scientific meetings.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

一个奖项是作出斯基德莫尔学院，以支持购买仪器测量微妙的，实时变化线粒体呼吸的两个主要输出测量：耗氧量和细胞外酸化。96孔自动格式允许以高精度和高分辨率对小样品体积进行高通量分析。该分析仪将被神经科学项目和生物学，化学和健康人类生理科学系的教师和学生用于基础科学研究，转化研究和教学。实验使用和数据分析将被整合到本科科学课程的多门课程中，从而吸引广泛的本科用户。此外，该仪器将在夏季研讨会上为斯基德莫尔学者在科学和数学（S3 M）。S3 M学者是正在崛起的学者，他们是来自STEM代表性不足背景的强大科学学生。预计通过课程和S3 M研讨会接触仪器和分析实践将引导学生在教师的指导下将仪器纳入学生驱动的独立研究中。该分析仪适用于各种样品，包括培养细胞，切除组织，细菌和小动物胚胎，使其成为斯基德莫尔教师各种研究兴趣的理想选择。PI和co-PI的直接研究用途包括研究神经元细胞中的线粒体功能，衰老对肌肉和内皮细胞的影响，C.线虫突变体，鱼鳃离子细胞代谢的内分泌控制的鉴定，以及细菌中金属稳态的研究。该仪器的收购恰逢斯基德莫尔学院新的综合科学中心开幕，这是一座新的科学大楼，将所有10个STEM部门和项目汇集在一起。重要的是，关于共享使用该仪器的知识价值符合学院的使命，即在科学中整合研究和教学。此外，该仪器将有助于斯基德莫尔科学家和外部研究人员之间现有的积极和未来的合作。新系统获得的数据将在同行评审的出版物和科学会议上传播。该奖项反映了NSF的法定使命，并通过使用基金会的知识价值和更广泛的影响审查标准进行评估，被认为值得支持。