MRI: Acquisition of Flexible High-speed Super-Resolution System for Plant Research

唐纳德·丹福斯植物科学中心（DDPSC）获得了一项奖项，该奖项旨在对植物进行高速和超分辨率显微镜观察，以了解它们与微生物及其环境的相互作用。DDPSC的使命是通过植物科学改善人类状况：为饥饿者提供食物和改善人类健康；保护和更新环境；加强圣路易斯地区作为世界植物科学中心的地位。DDPSC在先进显微镜上的投资极大地有利于其植物研究项目，并为更大的科学界提供了新的植物应用。这种超分辨率显微镜将用于培训各级科学家直至主要研究人员。圣路易斯大学和圣路易斯华盛顿大学的三门研究生/本科课程将在他们的教学大纲中包括超分辨率显微镜。未来的科学家将通过积极参与DDPSC nsf本科生研究经验（REU）计划，教授每年两次的高级荧光成像短期课程，为成像项目招募高中生，并参加一个名为“学生和教师作为研究科学家”（STARS）的高中生区域实习计划来培训。此外，非常成功的DDPSC教育和推广项目将为教师举办研讨会，并提供互动指导，将我们的科学家与附近的学校联系起来，包括圣查尔斯社区学院和哈里斯斯托大学，一所历史悠久的黑人学院和大学。近年来，得益于基因编码荧光传感器和其他设计探针的快速发展，关于细胞如何工作的新发现层出不穷。此外，新的成像技术和方案已经出现，导致如何探索细胞和组织的革命。DDPSC获得高速超分辨率显微镜将对23个资助研究项目产生直接影响，这些研究项目包括NSF（12）、DOE(4)、DARPA(1)、USDA(2)、private(3)、NIH(1)，代表丹佛斯中心和当地地区的13个pi，包括圣路易斯华盛顿大学（WUSTL）、圣路易斯大学（SLU）和堪萨斯州立大学（KSU）。该超分辨率显微镜将有助于研究植物发育、微生物、植物-微生物相互作用、植物和藻类中的热/胁迫反应、植物和藻类细胞生长和分裂的基础、细胞骨架动力学、植物细胞壁、核结构域结构和动力学、RNA定位和植物或藻类组织中的蛋白质定位。研究结果将在同行评议的出版物和科学会议上传播。该奖项反映了美国国家科学基金会的法定使命，并通过使用基金会的智力价值和更广泛的影响审查标准进行评估，认为值得支持。

An award is made to the Donald Danforth Plant Science Center (DDPSC) to do high-speed and super-resolution microscopy of plants to understand their interactions with microbes and their environment. The DDPSC mission is to improve the human condition through plant science: by feeding the hungry and improving human health; preserving and renewing the environment; and enhancing the St. Louis region as a world center for plant science. The investment in advanced microscopy at the DDPSC greatly benefit its plant research projects and provides novel plant focused applications for the greater scientific community. The super-resolution microscope will be leveraged for training scientists at all ranks up to principal investigators. Three graduate/undergraduate courses at Saint Louis University and Washington University in Saint Louis will include super-resolution microscopy in their syllabus. Tomorrow’s scientists will be trained through active participation in the DDPSC NSF-Research Experiences for Undergraduates (REU) Program, teaching a twice-yearly advanced fluorescence imaging short course, recruitment of high school students for imaging projects and participation in a regional internship program for high school students called Students and Teachers As Research Scientists (STARS). Additionally, the highly successful DDPSC Education and Outreach program will host workshops for teachers and provide interactive mentoring that connect our scientists with nearby schools including St. Charles Community College and Harris Stowe University, a Historically Black College and University.In recent years, a remarkable surge in new discoveries on how cells work have been forthcoming, benefiting from the rapid development of genetically encoded fluorescent sensors and other designer probes. Additionally, new imaging technologies and protocols have arisen, leading to a revolution in how a cells and tissues can be explored. DDPSC acquisition of a high-speed super-resolution microscope will have an immediate impact on twenty-three funded research projects NSF (12), DOE (4), DARPA (1) USDA (2), private (3), NIH (1) representing 13 PIs at the Danforth Center and local region including Washington University of Saint Louis (WUSTL), Saint Louis University (SLU) and Kansas State University (KSU). The super-resolution microscope will enable advancements in diverse projects which investigate plant development, microbes, plant-microbe interactions, heat/stress responses in plants and algae, fundamentals of plant and algal cell growth and division, cytoskeletal dynamics, the plant cell wall, nuclear domain structure and dynamics, RNA localization, and protein localization in plant or algal tissues. Results from the research will be disseminated in peer-reviewed publications and at scientific meetingsThis award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.