Role of Tic40 in Male Sterility in Oilseed Rape

Tic40 在油菜雄性不育中的作用

• 批准号: 243104960
• 负责人: Professor Dr. Jürgen Soll
• 金额: --
• 依托单位: Lehrstuhl für Biochemie und Physiologie der Pflanzen
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2013
• 资助国家: 德国
• 起止时间: 2012-12-31 至 2016-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/243104960?language=en
• 关键词: Role; Tic40; Male; Sterility; Oilseed

The endosymbiotic organelles mitochondria and plastids need to import most of their protein constituents from the cytosol in a posttranslational process. Multisubunit protein transport machineries named translocon at the outer and inner envelope of chloroplasts (TOC and TIC), respectively, are responsible for preprotein recognition and translocation. Tic40 is a prominent subunit of the translocon and localizes to the stromal side of the Tic complex, where it is supposed to recruit molecular chaperones to the exit site of preproteins from the translocation channel. A detailed physiological and functional analysis in the model plant Arabidopsis thaliana is, however, not possible since a loss of function mutant of the single Tic40 coding gene results in a severe pale green pleiotropic phenotype, which precludes specific analysis. In the related species Brassica napus (oilseed rape), multiple Tic40 gene product exist. One isoform BnaCTic40 was further shown to function as a restorer gene for male sterility in oilseed rape, thereby allowing to achieve two aims with this proposal project. Firstly, to get a detailed insight into the physiological and mechanistic function of Tic40. Secondly, we will understand the role of Tic40 in fertility restoration in oilseed rape, an important feature to improve the breeding process.

内共生细胞器、线粒体和质体需要在翻译后过程中从胞质溶胶中输入大部分蛋白质成分。位于叶绿体外被膜和内被膜上的多亚基蛋白质转运机构（TOC和TIC）分别负责前体蛋白的识别和转运。Tic40是易位子的一个重要亚基，定位于Tic复合物的基质侧，在那里它应该将分子伴侣从易位通道招募到前蛋白的出口位点。然而，在模式植物拟南芥中进行详细的生理和功能分析是不可能的，因为单个Tic40编码基因的功能缺失突变体导致严重的浅绿色多效性表型，这排除了特异性分析。在相关物种甘蓝型油菜（Brassica napus）（油菜）中，存在多个Tic40基因产物。一种同工型BnaCTic40进一步显示出作为油菜雄性不育恢复基因的功能，从而允许实现本提案项目的两个目的。首先，详细了解Tic40的生理和机制功能。其次，我们将了解Tic40在油菜育性恢复中的作用，这是改善育种过程的重要特征。