Collaborative Research: Protein engineering and processing of plant viral templates for controlled nanoparticle synthesis

合作研究：用于受控纳米颗粒合成的植物病毒模板的蛋白质工程和加工

• 批准号: 2028634
• 负责人: Shohreh Hemmati
• 金额: $ 21.16万
• 依托单位: Oklahoma State University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-09-15 至 2024-04-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2028634&HistoricalAwards=false
• 关键词: Collaborative; Research; Protein; engineering; processing

Nano-scaled materials have unique properties that are tied to their size. The properties of these materials are controlled by their architecture. As a result, their manufacture must be tightly controlled. A uniform template would be extremely useful in such processes. Barley stripe mosaic virus (BSMV) is a plant virus with highly uniform structure. Using templates derived from BSMV is proposed as an economical alternative to current processes. Virus-like particles (VLPs) derived from BSMV will be designed to act as templates for the production of metallic nanomaterials. Graduate and undergraduate students in will be trained to design and produce these templates. High school students from underrepresented groups and the general public will be engaged through innovative workshops that teach them about the power of biological engineering and nanomaterials. BSMV is an attractive biotemplate for synthesis of metallic nanomaterials. It can be more densely coated with metal than other biotemplates. It interacts electrostatically with metal precursor ion solutions to deposit a wider range of metals. It requires fewer processing steps to coat fully. The role of key BSMV amino acid residues in mediating metal mineralization on the VLP surface will be investigated. Protein and RNA engineering strategies to control biotemplate properties and facilitate biotemplate robustness will be pursued. Surface residues will be altered to facilitate metal-template interactions. Thermal processing to create biotemplated-materials with high electrical conductivities will be optimized. If successful, the techniques developed will create an economical bacterial expression and purification pipeline for designer BSMV biotemplates. The range of metals that ca be deposited on them will be broadened. Key insights into organic-inorganic interactions involved in metal deposition on biotemplates will be uncovered. The project results may elucidate structural details about how BSMV capsid proteins self-assemble, having implications for the infection and treatment of cereal crops that are susceptible to BSMV.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

纳米级材料具有与其尺寸相关的独特特性。这些材料的特性是由它们的结构控制的。因此，它们的生产必须受到严格控制。在这种过程中，统一的模板将非常有用。大麦条纹花叶病毒（BSMV）是一种结构高度均匀的植物病毒。使用BSMV衍生的模板被认为是当前流程的经济替代方案。从BSMV衍生的病毒样颗粒（VLPs）将被设计为生产金属纳米材料的模板。研究生和本科生将接受设计和制作这些模板的培训。来自代表性不足群体的高中生和普通大众将通过创新研讨会参与其中，向他们传授生物工程和纳米材料的力量。BSMV是一种有吸引力的金属纳米材料合成生物模板。它可以比其他生物模板更密集地包裹金属。它与金属前驱体离子溶液静电相互作用，沉积更广泛的金属。它需要更少的加工步骤来完全涂覆。研究BSMV关键氨基酸残基在VLP表面介导金属矿化中的作用。蛋白质和RNA工程策略，以控制生物模板的性质和促进生物模板的鲁棒性将继续追求。表面残基将被改变以促进金属-模板相互作用。将优化热加工以制造具有高导电性的生物模板材料。如果成功，该技术将为设计BSMV生物模板创建一个经济的细菌表达和纯化管道。可以沉积在上面的金属的范围将会扩大。将揭示生物模板上金属沉积中有机-无机相互作用的关键见解。该项目的结果可能阐明BSMV衣壳蛋白如何自组装的结构细节，对易受BSMV感染的谷类作物的感染和治疗具有指导意义。该奖项反映了美国国家科学基金会的法定使命，并通过使用基金会的知识价值和更广泛的影响审查标准进行评估，被认为值得支持。