RUI: Investigating the pathways and regulation of lysosome-related organelle biogenesis.
RUI:研究溶酶体相关细胞器生物发生的途径和调节。
基本信息
- 批准号:2051826
- 负责人:
- 金额:$ 54.99万
- 依托单位:
- 依托单位国家:美国
- 项目类别:Standard Grant
- 财政年份:2021
- 资助国家:美国
- 起止时间:2021-07-15 至 2024-06-30
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
The project will discover how intracellular compartments are formed during embryonic development. Animal cells contain compartments called organelles that often gives individual cells their specialized function. One of these compartments, called a lysosome-related organelle (LRO), mediates important activities, including the creation of body pigments, the storage of nutrients, and signals for aging. This project will discover when these organelles are first created during animal development, investigate how material is directed to these compartments, and study how their number and size are regulated. Each year, 30-40 undergraduates will be mentored in collaborative, investigative, and original research as part of the project. The project will provide hands on scientific training for a large and diverse group of students, most of who will likely progress on to careers in science and education.Little is currently known about how the trafficking pathways to LROs emerge during cellar differentiation, whether endolysosomes, such as late endosomes or lysosomes, function in LRO protein delivery, and how biogenesis pathways are regulated to control LRO number and size. To investigate these processes, this project analyzes the biogenesis of gut granules, LROs found in C. elegans intestinal cells. The research will use genetic approaches to examine whether LRO biogenesis genes function in the germline or embryo, and after determining when LRO proteins are first made during development, evaluate their distribution relative to endosomal organelle markers using quantitative fluorescence microscopy. In addition, whether gut granule cargo is routed through endolysosomes will be using pulse chase and add-back approaches, as well as live-cell fluorescence imaging of interactions between endolysosomes and gut granules. When pathways directing LRO protein trafficking are active will be determined by tracking gut granule cargo following its targeted expression at different stages of development combined with newly developed methods to potently disrupt the function of LRO biogenesis proteins. Finally, a collection of C. elegans mutants with enlarged gut granules will be assessed to identify potential regulators of LRO homeostasis.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.
该项目将发现胚胎发育过程中细胞内隔室是如何形成的。 动物细胞包含称为细胞器的隔室,这些隔室通常赋予单个细胞专门的功能。 其中一个被称为溶酶体相关细胞器(LRO),介导重要的活动,包括身体色素的产生,营养物质的储存和衰老信号。 该项目将发现这些细胞器在动物发育过程中何时首次产生,研究材料如何被引导到这些隔室,并研究它们的数量和大小如何被调节。 作为该项目的一部分,每年将有30-40名本科生接受协作,调查和原创研究的指导。该项目将为一个庞大而多样化的学生群体提供科学培训,其中大多数人可能会在科学和教育方面取得进展。目前对LRO的运输途径如何在细胞分化过程中出现知之甚少,无论是内溶酶体,如晚期内体或溶酶体,在LRO蛋白质递送中发挥作用,以及如何调节生物合成途径以控制LRO数量和大小。为了研究这些过程,本项目分析了肠道颗粒的生物发生,在C。肠细胞 该研究将使用遗传学方法来检查LRO生物发生基因是否在种系或胚胎中起作用,并在确定LRO蛋白在发育过程中何时首次产生后,使用定量荧光显微镜评估它们相对于内体细胞器标记物的分布。 此外,肠道颗粒货物是否通过内溶酶体路由将使用脉冲追逐和添加回方法,以及内溶酶体和肠道颗粒之间的相互作用的活细胞荧光成像。 当引导LRO蛋白运输的途径是活跃的时,将通过跟踪肠道颗粒货物在不同发育阶段的靶向表达结合新开发的方法来确定,以有效地破坏LRO生物合成蛋白的功能。最后,收集了C.该奖项反映了NSF的法定使命,并被认为是值得通过使用基金会的智力价值和更广泛的影响审查标准进行评估的支持。
项目成果
期刊论文数量(1)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Greg Hermann其他文献
Greg Hermann的其他文献
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{{ truncateString('Greg Hermann', 18)}}的其他基金
RUI: Investigating the mechanisms regulating the formation of lysosome-related organelles
RUI:研究调节溶酶体相关细胞器形成的机制
- 批准号:
1613804 - 财政年份:2016
- 资助金额:
$ 54.99万 - 项目类别:
Standard Grant
RUI-Investigating the Mechanism of GLO-1 Rab Function in C. Elegans Lysosome-related Organelle Biogenesis
RUI-研究线虫溶酶体相关细胞器生物发生中 GLO-1 Rab 功能的机制
- 批准号:
1120835 - 财政年份:2011
- 资助金额:
$ 54.99万 - 项目类别:
Continuing Grant
MRI: Acquisition of a Fluorescence Deconvolution Microscope For Research And Training at Lewis & Clark College
MRI:购买荧光解卷积显微镜用于刘易斯研究和培训
- 批准号:
0922613 - 财政年份:2009
- 资助金额:
$ 54.99万 - 项目类别:
Standard Grant
Cellular and Genetic Analysis of Lysosome and Lysosome-Related Organelle Biogenesis in C. Elegans
线虫溶酶体和溶酶体相关细胞器生物发生的细胞和遗传分析
- 批准号:
0716280 - 财政年份:2007
- 资助金额:
$ 54.99万 - 项目类别:
Continuing Grant
RUI: Genetic and Molecular Analysis of Lysosome Assembly and Stability in C. Elegans
RUI:线虫溶酶体组装和稳定性的遗传和分子分析
- 批准号:
0314332 - 财政年份:2003
- 资助金额:
$ 54.99万 - 项目类别:
Continuing Grant
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