Protein-recognition by supramolecular ligands from focused combinatorial libraries (A01)

来自聚焦组合文库的超分子配体的蛋白质识别 (A01)

• 批准号: 247858263
• 金额: --
• 依托单位: Organische Chemie - AG Supramolekulare Chemie
• 依托单位国家: 德国
• 项目类别: Collaborative Research Centres
• 财政年份: 2014
• 资助国家: 德国
• 起止时间: 2013-12-31 至 2020-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/247858263?language=en
• 关键词: Protein; recognition; supramolecular; ligands; focused

In project A1 we develop multi-armed peptide based ligands for proteins in order to modulate their function (e.g. by interfering with essential protein protein interactions). The ligands contain non-natural amino acids with tailor-made specific supramolecular binding sites, such as our guanidiniocarbonyl pyrrole cation as a highly efficient anion binder. The ligands therefore target anionic hot spots on the proteins. Ligand design is based on available structural information of the target and for optimization combinatorial approaches such as the screening of dynamic combinatorial libraries is used.

在项目A1中，我们开发了基于多臂肽的蛋白质配体，以调节其功能（例如通过干扰必需的蛋白质相互作用）。配体含有非天然氨基酸，具有定制的特异性超分子结合位点，例如我们的胍基羰基吡咯阳离子作为高效阴离子结合剂。因此，配体靶向蛋白质上的阴离子热点。配体设计基于靶的可用结构信息，并且为了优化，使用组合方法，例如筛选动态组合文库。