Protein-recognition by supramolecular ligands from focused combinatorial libraries (A01)

在A1项目中，我们开发了基于多肽的蛋白质配体，以调节它们的功能（例如，通过干扰必需的蛋白质相互作用）。这些配体含有非天然氨基酸，具有定制的特定超分子结合位点，例如我们的胍基羰基吡咯阳离子是一种高效的阴离子结合剂。因此，配体以蛋白质上的阴离子热点为目标。配体设计是基于目标的可用结构信息和优化组合方法，如筛选动态组合文库被使用。

In project A1 we develop multi-armed peptide based ligands for proteins in order to modulate their function (e.g. by interfering with essential protein protein interactions). The ligands contain non-natural amino acids with tailor-made specific supramolecular binding sites, such as our guanidiniocarbonyl pyrrole cation as a highly efficient anion binder. The ligands therefore target anionic hot spots on the proteins. Ligand design is based on available structural information of the target and for optimization combinatorial approaches such as the screening of dynamic combinatorial libraries is used.