Role of lipids in membrane protein insertion (22)

脂质在膜蛋白插入中的作用 (22)

• 批准号: 251938480
• 金额: --
• 依托单位: Biochemie-Zentrum (BZH)
• 依托单位国家: 德国
• 项目类别: CRC/Transregios
• 财政年份: 2014
• 资助国家: 德国
• 起止时间: 2013-12-31 至 2020-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/251938480?language=en
• 关键词: Role; lipids; membrane; protein; insertion

The project aims at the structural and functional characterization of the receptor proteins of the GET pathway, Get1 and Get2, which are integral membrane proteins. We showed that Get1/2 can form a dimer of the heterodimer, which contains a particular lipid species. We now plan to determine the structure of the GET receptor using a hybrid approach combining X-ray crystallography and electron microscopy, and specifically study the role of lipids on its structure and function. Using cross-linking and MS with specifically modified lipids we will analyze their functional implications for Get3 recruitment and tail-anchored membrane protein insertion. With the combination of structural and biochemical assays we will arrive at a detailed understanding of the structure and mechanism of the Get1/2/3 insertase and dissect the membrane associated steps of the GET pathway.

该项目的目的是GET途径的受体蛋白Get 1和Get 2的结构和功能特性，这是一种不可或缺的膜蛋白。我们发现，Get 1/2可以形成异二聚体的二聚体，其中含有特定的脂质种类。我们现在计划使用X射线晶体学和电子显微镜相结合的混合方法来确定GET受体的结构，并专门研究脂质对其结构和功能的作用。使用交联和MS与专门修饰的脂质，我们将分析其功能的影响Get 3招聘和尾锚定膜蛋白插入。结合结构和生化分析，我们将详细了解Get 1/2/3插入酶的结构和机制，并剖析GET途径的膜相关步骤。