MRI: Acquisition of a Microscale Thermophoresis System

MRI：获取微型热泳系统

• 批准号: 2215022
• 负责人: Amanda Hargrove
• 金额: $ 18.18万
• 依托单位: Duke University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-08-15 至 2025-07-31
• 项目状态: 未结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2215022&HistoricalAwards=false
• 关键词: MRI; Acquisition; Microscale; Thermophoresis; System

This award is jointly supported by the Major Research Instrumentation and the Chemistry Research Instrumentation Programs. Duke University is acquiring a microscale thermophoresis system to advance the research of Professor Amanda Hargrove and colleagues. This instrument facilitates research in the areas of chemistry, biochemistry, and chemical biology. Microscale thermophoresis directly measures molecular movement in a temperature gradient to quantify molecular interactions. Signals are quantified by measuring fluorescence arising from labeling or intrinsic properties. The resulting measurements provide structural and functional characterization of interactions between biological, inorganic, and organic molecules. This instrument enhances the educational, research, and teaching efforts of students at all levels in many departments as well as provides accessibility for use at nearby institutions. Notably, the instrument would be leveraged in undergraduate physical chemistry laboratory courses where students gain hands on experience with the instrument.The award for the microscale thermophoresis system is aimed at enhancing research and education at all levels, especially in areas such as organic and inorganic chemistry, chemical biology, biopolymers, and biochemistry. Research focuses on measuring binding interactions between RNA and small molecules, peptides and metal ions, and other biomolecules and small molecules. In addition, domain-domain interactions in enzymes, proteins, and cells are pursued.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

该奖项由主要的研究工具和化学研究工具计划共同支持。杜克大学（Duke University）正在收购微观嗜热系统，以推动阿曼达·哈格罗夫（Amanda Hargrove）及其同事的研究。该仪器促进了化学，生物化学和化学生物学领域的研究。微观热疗法直接测量温度梯度中的分子运动，以量化分子相互作用。通过测量由标记或固有特性引起的荧光来量化信号。所得的测量值提供了生物，无机和有机分子之间相互作用的结构和功能表征。该工具增强了许多部门各级学生的教育，研究和教学工作，并为附近机构提供了可访问性。值得注意的是，该仪器将在本科生的化学实验室课程中利用该仪器，在该课程中，学生可以在该乐器上获得经验。微观嗜热系统奖旨在增强各个级别的研究和教育，尤其是在有机和无机化学，化学化学，生物学，生物聚合物和生物化学的领域。研究重点是测量RNA与小分子，肽和金属离子以及其他生物分子和小分子之间的结合相互作用。此外，追求酶，蛋白质和细胞中的域域相互作用。该奖项反映了NSF的法定任务，并认为使用基金会的知识分子优点和更广泛的影响评估标准，被认为值得通过评估。