MRI: Acquisition of a Biolayer Interferometry Spectrometer to Measure Macromolecule Binding Interactions

MRI：获取生物层干涉光谱仪来测量大分子结合相互作用

• 批准号: 2215642
• 负责人: Dominic Qualley
• 金额: $ 10.85万
• 依托单位: Berry College
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-08-15 至 2025-07-31
• 项目状态: 未结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2215642&HistoricalAwards=false
• 关键词: MRI; Acquisition; Biolayer; Interferometry; Spectrometer

This award is jointly supported by the Major Research Instrumentation and the Chemistry Research Instrumentation Programs. Berry College is acquiring a bio-layer interferometry (BLI) spectrometer to support the research of Professor Dominic Qualley and colleagues Angela Poole, Alice Suroviec, and Mark Turlington. This instrument facilitates research in the areas of macromolecular binding kinetics and real-time biomolecular interactions. BLI is an optical method that measures interference patterns of white light that are reflected from a surface coated in biomolecules to reveal real-time binding interactions. As a result, macromolecular interactions, affinity, and kinetics can be quantified. This instrument enhances the educational, research, and teaching efforts of students in many departments as well as provides accessibility for local middle and high school students. Research and research training in the areas of biotechnology and sustainability are targeted. The instrument is also leveraged in upper division laboratory undergraduate courses and in outreach activities for middle and high school students.The award of the BLI spectrometer is aimed at enhancing research and education at all levels, especially in areas such as protein structure and function, bioanalytical and medicinal chemistry, and functional biology. Research focuses on understanding the structure and function of proteins involved in viral assembly. In addition, kinetics studies are performed for characterizing the bovine leukemia virus RNA packaging processes and the assembly mechanics of the West Nile virus as well as for elucidating parameters that are effective proteolysis targeting chimeras. It also assists with exploring protein-protein interactions for biosensor development and for investigating interactions associated with reproductive technologies in animal systems.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

该奖项由主要研究仪器和化学研究仪器计划共同支持。贝瑞学院正在购买生物层干涉（BLI）光谱仪，以支持Dominic Qualley教授及其同事Angela Poole，Alice Suroviec和Mark Turlington的研究。该仪器有助于大分子结合动力学和实时生物分子相互作用领域的研究。BLI是一种光学方法，其测量从生物分子涂覆的表面反射的白色光的干涉图案，以揭示实时结合相互作用。因此，可以量化大分子相互作用、亲和力和动力学。该工具增强了许多部门学生的教育，研究和教学工作，并为当地初中和高中学生提供了无障碍环境。目标是生物技术和可持续性领域的研究和研究培训。该仪器还被用于高年级实验室本科课程和面向初中和高中学生的推广活动。BLI光谱仪的获奖旨在加强各级研究和教育，特别是在蛋白质结构和功能，生物分析和药物化学以及功能生物学等领域。研究重点是了解参与病毒组装的蛋白质的结构和功能。此外，动力学研究进行表征牛白血病病毒RNA包装过程和西尼罗河病毒的组装力学，以及阐明参数，是有效的蛋白水解靶向嵌合体。它还有助于探索生物传感器开发的蛋白质-蛋白质相互作用，以及研究与动物系统中生殖技术相关的相互作用。该奖项反映了NSF的法定使命，并通过使用基金会的知识价值和更广泛的影响审查标准进行评估，被认为值得支持。