MRI: Acquisition of a FACSMelody Flow Cytometer and Cell Sorter

MRI：购买 FACSMelody 流式细胞仪和细胞分选仪

• 批准号: 2216005
• 负责人: Kinta Serve
• 金额: $ 26.3万
• 依托单位: Idaho State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-10-01 至 2025-09-30
• 项目状态: 未结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2216005&HistoricalAwards=false
• 关键词: MRI; Acquisition; FACSMelody; Flow; Cytometer

An award is made to Idaho State University to acquire a Fluorescence-Activated Cell Sorter (FACS) flow cytometer. This instrument will greatly enhance the research and educational opportunities across multiple departments and colleges at Idaho State University and at near-by undergraduate institution BYU-I. The instrument will be housed in a multi-user core facility and accessed by researchers and teachers in the Biological Sciences, Pharmaceutical Sciences, and Anthropology. Hands-on experimental design modules will be developed and utilized in various undergraduate and graduate courses, and within secondary education teacher-training courses. By incorporating this equipment into classes, students at all levels of education will gain hands-on experience in authentic research activities. This equipment will also be accessible to community partners and incorporated into university-wide outreach and STEM training activities to promote recruitment, retention, and professional development of a diverse workforce.This instrument will be utilized by a variety of researchers, from undergraduate students to professors. The ease of instrument set-up and diversity of applications across multiple disciplines will enhance current research capabilities and open opportunities for new and innovative projects. Researchers will use this machine to answer questions pertaining to 1) immune cell responses to environmental contaminants and physiologic stimuli, 2) cellular protein expression during organismal and cellular development, 3) plant genome/ploidy quantification, 4) hormone receptor expression in animal tissues, 5) gene expression in prokaryotes, 6) cell division and death, and 7) microparticle identification in archeologic samples (i.e., teeth, pottery). Many of these areas rely on the sorting functions of this machine to isolate live cells or small particles for further experimentation. Data generated using this instrument will be disseminated to the public via presentations and peer-reviewed papers, and data will impact a wide array of fields while providing cutting-edge training for students in environmental sciences, developmental biology, evolutionary biology, eukaryotic and prokaryotic cell biology, and anthropology.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

爱达荷州州立大学获得了一个奖项，以获得一个荧光激活细胞分选仪（FACS）流式细胞仪。这一工具将大大提高跨多个部门和学院的研究和教育机会在爱达荷州州立大学和附近的本科院校杨百翰大学-I。该仪器将被安置在一个多用户的核心设施，并在生物科学，药学和人类学的研究人员和教师访问。将在各种本科和研究生课程以及中等教育教师培训课程中开发和使用实践实验设计模块。通过将这种设备纳入课堂，各级教育的学生将获得真实研究活动的实践经验。该设备也将提供给社区合作伙伴，并纳入大学范围内的外联和STEM培训活动，以促进招聘，保留和多元化劳动力的专业发展。该仪器将被各种研究人员使用，从本科生到教授。仪器设置的简便性和跨多学科应用的多样性将增强当前的研究能力，并为新的创新项目提供机会。研究人员将使用这台机器回答有关以下问题：1）免疫细胞对环境污染物和生理刺激的反应，2）生物体和细胞发育期间的细胞蛋白质表达，3）植物基因组/倍性定量，4）动物组织中的激素受体表达，5）原核生物中的基因表达，6）细胞分裂和死亡，和7）考古样品中的微粒识别（即，牙齿、陶器）。这些领域中的许多都依赖于这台机器的分选功能来分离活细胞或小颗粒，以供进一步实验。使用该仪器生成的数据将通过演示和同行评审论文向公众传播，数据将影响广泛的领域，同时为学生提供环境科学，发育生物学，进化生物学，真核和原核细胞生物学，该奖项反映了NSF的法定使命，并被认为是值得通过使用基金会的智力价值和更广泛的影响审查标准。