Role and activity of PRC2 and SWI/SNF in plant adaptability to changing environments

PRC2 和 SWI/SNF 在植物适应不断变化的环境中的作用和活性

• 批准号: 2224729
• 负责人: Doris Wagner
• 金额: $ 100万
• 依托单位: University of Pennsylvania
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-08-15 至 2025-07-31
• 项目状态: 未结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2224729&HistoricalAwards=false
• 关键词: Role; activity; PRC2; SWI; SNF

Drought or heat cause dramatic crop losses each year. Plants can adjust to adverse conditions by activating different regulatory programs. This requires changes to the epigenome. The research proposed will elucidate how the epigenome is altered when drought or heat stress is sensed by the plant. Insight gained will enable optimization of plant growth and reduction of the detrimental effects of stress. In addition, the proposal will train undergraduate students in course-based laboratory research to conduct original research that contributes to addressing the goals of this proposal, to enhance plant productivity via epigenome change.To elucidate the contribution of PRC2 repression and SWI/SNF chromatin remodeling to plant adaptability to environmental challenges, this proposal will rapidly deplete either epigenetic regulator, followed by assessing immediate early effects on the chromatin state and gene expression, as well as phenotypic response to environmental challenge. This will generate novel insight into direct biological contribution roles of SWI/SNF and PRC2 to plant adaptability to changing environments. In parallel, the contribution of individual chromatin regulators to overcoming the existing chromatin state will be assessed. In doing so, the project will generate novel tools for the plant community to dissect direct roles and activities of key regulators. The broader impacts of this proposal include insight into how plant resilience to changing environments is facilitated via epigenome reprogramming. In addition, three one-semester course-based undergraduate research experience (CURE) laboratory classes will be conducted. The undergraduate researchers will define the contact domains between SWI/SNF or PRC2 and transcription factors to shed light on the types contact ‘surfaces’ that enable chromatin reprogramming. Finally, lectures on research conducted in this proposal will be given to high school students during the yearly summer research academy.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

干旱和高温每年都会造成巨大的农作物损失。植物可以通过激活不同的调节程序来适应不利条件。这需要改变表观基因组。这项研究将阐明当植物感受到干旱或热胁迫时，表观基因组是如何改变的。获得的见解将使植物生长的优化和减少压力的不利影响。此外，该提案将在基于课程的实验室研究中培养本科生进行有助于实现该提案目标的原创性研究，通过表观基因组变化提高植物生产力。为了阐明PRC 2抑制和SWI/SNF染色质重塑对植物适应环境挑战的贡献，该提案将迅速耗尽表观遗传调节因子，随后评估对染色质状态和基因表达的即时早期效应，以及对环境挑战的表型反应。这将产生新的见解直接生物贡献作用的SWI/SNF和PRC 2植物适应不断变化的环境。同时，将评估单个染色质调节剂对克服现有染色质状态的贡献。在这样做的过程中，该项目将为植物社区提供新的工具，以剖析关键监管机构的直接作用和活动。这一提议的更广泛影响包括深入了解植物如何通过表观基因组重编程促进对不断变化的环境的适应力。此外，三个一学期的课程为基础的本科研究经验（CURE）实验室类将进行。本科生研究人员将定义SWI/SNF或PRC 2与转录因子之间的接触域，以揭示使染色质重编程的接触“表面”类型。最后，在每年的夏季研究会期间，将向高中生提供关于本提案中的研究的讲座。该奖项反映了NSF的法定使命，并通过使用基金会的知识价值和更广泛的影响审查标准进行评估，被认为值得支持。