Structure-function analysis of nuclear export complexes and their interaction with nucleoporins (B08)

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CRM1 is the major nuclear export receptor for proteins and also for a subset of RNAs. In the first part of this project, we plan to analyse the significance of the interaction of CRM1 with an NES-like sequence in the nucleoporin Nup214 for nuclear transport and also for the biogenesis of the nuclear pore complex. In the second part, we want to investigate CRM1-containing RNA-export complexes by means of single-particle cryo-electron microscopy (cryo-EM), X-ray crystallography and mass spectrometry.