Collaborative Research: RAPID: Genomic and phenotypic responses to hurricane-mediated selection in an invasive lizard: does epistasis constrain evolution?

合作研究：RAPID：入侵蜥蜴对飓风介导的选择的基因组和表型反应：上位性是否限制进化？

• 批准号: 2349094
• 负责人: Sean Giery
• 金额: $ 9.12万
• 依托单位: Ohio University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-10-01 至 2024-10-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2349094&HistoricalAwards=false
• 关键词: Collaborative; Research; RAPID; Genomic; phenotypic

This project will study evolutionary responses to extreme weather events and increase our understanding of how genetics influences a population’s response to selection. We know that extreme weather events such as hurricanes can have long-lasting, evolutionary effects on wild populations. However, no information exists about how genomic factors that might facilitate or impede that evolution.The project will focus on brown anoles (Anolis sagrei), a widespread invasive lizard in Florida that is native to Cuba. It will compare populations sampled in 2018 to those same populations following Hurricane Ian. Information on evolutionary responses (or lack thereof) to increasingly intense and frequent catastrophic weather will be a key component of our understanding of the response of wild populations to global change. The project will also train graduate and undergraduate students in field methods, as well as morphological and genomic and data analyses.Prior work consisted of high-quality genomic, habitat use, and morphological data from brown anoles. Sampling covered 30 populations distributed along the latitudinal extent of Florida and into southern Georgia. Guided by previous research, it is hypothesized that the strength of selection will be correlated with wind speeds, such that stronger winds will favor longer limbs. In addition, the evolutionary response to limb-length selection will be strongly affected by an interaction between a large-effect limb-length locus and the genomic background of brown anole populations. Specifically, there will be a weaker evolutionary response to hurricane-mediated selection on limb length in populations with extensive hybrid ancestry. Previous research found epistatic interactions between an otherwise large-effect locus for limb length and the genomic background for hybrid populations. Because these genomic backgrounds vary across Florida, these effects should vary geographically.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

该项目将研究对极端天气事件的进化反应，并提高我们对遗传学如何影响人群对选择的反应的理解。我们知道，诸如飓风等极端天气事件可能会对野生人群产生长期的进化影响。但是，尚无有关如何促进或阻碍该进化的基因组因素的信息。该项目将集中于棕色肛门（Anolis Sagrei），这是佛罗里达州的广泛侵入性蜥蜴，是古巴原生的。它将将2018年采样的人口与飓风伊恩之后的同一人口进行比较。关于进化反应（或缺乏进化反应）对日益强烈和经常灾难性天气的信息将是我们对野生种群对全球变化反应的理解的关键组成部分。该项目还将培训现场方法的毕业生和本科生，以及形态学和基因组和数据分析。采样涵盖了沿佛罗里达州纬度范围内和佐治亚州南部的30种人群。在先前的研究的指导下，假设选择的强度将与风速相关，从而使更强风将有利于较长的肢体。此外，对肢体长度选择的进化反应将受到大效应的肢体座位与棕色Anole种群的基因组背景之间的相互作用的强烈影响。具体而言，在具有广泛的混合血统的种群中，对飓风介导的肢体长度的选择将有较弱的进化反应。先前的研究发现，肢体长度的原本大效应基因座与杂种种群的基因组背景之间的同志相互作用。由于这些基因组背景在整个佛罗里达州各不相同，因此这些影响应在地理上有所不同。该奖项反映了NSF的法定任务，并通过使用基金会的知识分子优点和更广泛的影响评估标准来诚实地通过评估来诚实地支持。