Cytocompatibility and bioactivity testing in vitro

体外细胞相容性和生物活性测试

• 批准号: 270264384
• 负责人: Dr. Birgit Weyand
• 金额: --
• 依托单位: Department für Biotechnologie (DBT)
• 依托单位国家: 德国
• 项目类别: Research Units
• 财政年份: 2015
• 资助国家: 德国
• 起止时间: 2014-12-31 至 2019-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/270264384?language=en
• 关键词: Cytocompatibility; bioactivity; testing; vitro

Aim of this subproject is the development of a standardized cell-based assay method in or-der to test the suitability of new implant materials For the test procedure we will use mesenchymal stem cells in order to evaluate the biocompatibility of the material components. Furthermore, we will develop strategies to improve cell seeding and attachement, as well as static and dynamic culture techniques together with biomechanical stimulation of the cell-matrix-constructs in order to mimic physiological culture conditions.Both groups will isolate and characterise mesenchymal stem cells using identical protocols. In order to achieve sufficient cell numbers for testing, dynamic expansion techniques such as the Wave technology (cellbag bioreactors on mixing systems) will be established and optimized. Be-sides of microcarrier-based technologies we intend to use also particle-free microaggregate culture techniques. Besides we will study the influence of hypoxia (5% O2) on proliferation, differentiation and senescence of mesenchymal stem cells.Furthermore we will work on strategies to optimize cell seeding and attachment on different materials. Beside of cellular attachment to surface properties we will study the cellular ingrowth in-side the matrices. The cell-seeded constructs will then be cultured under dynamic conditions in specialized bioreactor systems and differentiated into the particular tissue types, such as tendon or bone, for functional testing of the implant. In this part of the project we will concentrate on protein-functionalized matrices from TP1, TP2, and TP5 seeded with mesenchymal stem cells and we will test the effects of proteins on cell differentiation. In parallel, we will test impact and efficiency of different sterilization methods in static long-term cultures.

这个子项目的目的是开发一种标准化的基于细胞的检测方法，以测试新植入材料的适合性。我们将使用间充质干细胞来评估材料成分的生物相容性。此外，我们将开发改进细胞接种和贴壁的策略，以及静态和动态培养技术，以及对细胞基质结构的生物机械刺激，以模拟生理培养条件。两组将使用相同的方案分离和鉴定间充质干细胞。为了获得足够的细胞数量以进行测试，将建立和优化动态扩张技术，如Wave技术(混合系统上的纤维袋生物反应器)。除了基于微载体的技术外，我们还打算使用无颗粒微聚体培养技术。此外，我们还将研究低氧(5%O2)对间充质干细胞增殖、分化和衰老的影响。此外，我们还将研究优化细胞在不同材料上的接种和附着的策略。除了细胞对表面性质的附着外，我们还将研究细胞在基质中的生长。然后，细胞种植的结构将在专门的生物反应器系统中在动态条件下培养，并分化为特定的组织类型，如肌腱或骨，用于植入物的功能测试。在该项目的这一部分，我们将集中在TP1、TP2和TP5中种植间充质干细胞的蛋白质功能化基质，并测试蛋白质对细胞分化的影响。同时，我们将测试不同灭菌方法在静态长期培养中的影响和效率。