Determination of Drugs in Dental Material

在滥用药物或药物后，这些物质会永久储存在牙齿硬组织（牙釉质、牙本质、龋齿物质）中，并暂时储存在牙齿表面的生物膜中。该项目的目的是调查外来物质进入牙齿硬组织的情况，并分析非矿化生物膜（牙菌斑）和矿化生物膜（牙结石），以缩短尿液和毛发药物检测窗口之间的间隔。模型物质（不同的滥用药物和药物）将被应用于与不同牙齿组织结合的接触时间和浓度之间的关联。还将评估唾液和死后变化对组织的影响。药物的吸收将在体外用牛牙本质和牙釉质样品模拟生理ph过程，并在临床研究中用口腔内样品支架模拟。对死者的几种不同牙科材料（牙本质、牙釉质、龋齿组织、生物膜）进行分析，并与血清、尿液、胆汁、毛发和其他组织的毒理学标准分析进行比较。所有牙齿组织将通过液相色谱-串联质谱（LC-MS/MS）的先进方法进行定性和定量分析。高分辨率飞行时间质谱法（LC-QTOF-MS）将应用于牙组织的死后药物筛选。本研究收集了新的见解，在窗口的检测药物在牙齿中，能够通过分析牙组织前药物摄取得出结论。

After the consumption of drugs of abuse or pharmaceuticals, the substances are stored permanently in dental hard tissue (enamel, dentin, carious material) and temporarily in biofilm on the surface of the teeth. The aims of project are the investigation of the incorporation of foreign substances into dental hard tissues and the analysis of non mineralised biofilm (dental plaque) and mineralised biofilm (dental calculus) to close the interval between the windows of drug detection of urine and hair. Model substances (different drugs of abuse and pharmaceuticals) will be applied to correlate the contact time and concentration on the incorporation into the different dental tissues. The influence of saliva and post-mortem changes on the tissues will also be evaluated. The uptake of drugs will be simulated in vitro with bovine dentin and enamel samples by mimicking the physiological pH-course, and in clinical studies with intra-oral sample holders. Several different dental materials (dentin, enamel, carious tissue, biofilm) of deceased shall be analysed and compared with the toxicological standard analysis in serum, urine, bile, hair, and other tissues. All dental tissues will be analysed qualitatively and quantitatively by advanced methods of liquid chromatography-tandem mass spectrometry (LC-MS/MS). High resolution Time-of-Flight mass spectrometric methods (LC-QTOF-MS) will be applied for post mortem drug screening in dental tissues.This study gathers new insights in the windows of detection of drugs in teeth to be able to conclude on a preceding drug uptake by analysis of dental tissues.