Signaling mechanism, subcellular localization and regulation of guanylyl cyclases A and B, the receptors for atrial and C-type natriuretic peptides (C07)

心房肽（ANP）和C型利钠肽（CNP）的心血管作用由两个跨膜环GMP形成鸟苷酸环化酶（GC）受体GC-A和GC-B介导。为了表征这些受体依赖于配体激活环鸟苷酸产生的机制，我们将使用FRTE-FlAsH方法并可视化活细胞中配体诱导的构象变化的动态。此外，我们将应用单分子显微镜和超分辨率成像来比较健康和肥大心肌细胞中GC-A和GC-B的不同亚细胞定位。

The cardiovascular actions of atrial (ANP) and C-type natriuretic peptides (CNP) are mediated by two transmembrane cyclic GMP forming guanylyl cyclase (GC) receptors, GC-A and GC-B. To characterize the mechanism of ligand-dependent activation of cGMP production by these receptors we will use a FRET-FlAsH approach and visualize the dynamics of ligand-induced conformational changes in living cells. In addition we will apply single-molecule microscopy and superresolution imaging to compare the distinct subcellular localizations of GC-A and GC-B in healthy and hypertrophied cardiomyocytes.