Deciphering fast intra- and intermolecular dynamics of G protein-coupled receptors by Fluorescence (Cross) Correlation Spectroscopy (A09 [C06])

该项目旨在分析GPCR激活的结构动力学，似乎发生在一个简单的开关模型之外。我们已经建立了一个荧光工具箱，用于基于荧光共振能量转移和荧光相关光谱的活细胞分析，使我们能够专注于先前预测的快速亚毫秒激活步骤，这是传统显微镜方法难以获得的。这些结果有望为GPCR激活的分子间和分子内动力学提供有价值的信息，特别是多种状态的存在及其转变动力学。

This project aims to analyze the structural dynamics of GPCR activation that seem to occur beyond a simple on-off model. We have set up a fluorescence toolbox for live cell analysis based on Fluorescence Resonance Energy Transfer and Fluorescence Correlation Spectroscopy that allows us to focus on previously predicted, fast submillisecond activation steps that are difficult to access by conventional microscopy methods. The results are expected to provide valuable information about the inter- and intramolecular dynamics of GPCR activation, notably the existence of multiple states and the kinetics of their transitions.