Deciphering fast intra- and intermolecular dynamics of G protein-coupled receptors by Fluorescence (Cross) Correlation Spectroscopy (A09 [C06])

通过荧光（交叉）相关光谱破译 G 蛋白偶联受体的快速分子内和分子间动力学 (A09 [C06])

• 批准号: 279230892
• 金额: --
• 依托单位: Rudolf-Boehm-Institut für Pharmakologie und Toxikologie
• 依托单位国家: 德国
• 项目类别: CRC/Transregios
• 财政年份: 2015
• 资助国家: 德国
• 起止时间: 2014-12-31 至 2019-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/279230892?language=en
• 关键词: Deciphering; fast; intra; intermolecular; dynamics

This project aims to analyze the structural dynamics of GPCR activation that seem to occur beyond a simple on-off model. We have set up a fluorescence toolbox for live cell analysis based on Fluorescence Resonance Energy Transfer and Fluorescence Correlation Spectroscopy that allows us to focus on previously predicted, fast submillisecond activation steps that are difficult to access by conventional microscopy methods. The results are expected to provide valuable information about the inter- and intramolecular dynamics of GPCR activation, notably the existence of multiple states and the kinetics of their transitions.

该项目旨在分析GPCR激活的结构动力学，这些动力学似乎超出了简单的开关模型。我们已经建立了一个荧光工具箱，用于基于荧光共振能量转移和荧光相关光谱的活细胞分析，使我们能够专注于以前预测的，快速的亚毫秒激活步骤，这些步骤很难通过传统的显微镜方法获得。预计这些结果将提供有价值的信息间和分子内的GPCR激活的动力学，特别是存在的多个状态和动力学的过渡。