Proteomic platform

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The proteomic platform will be an integral part of the research consortium and performs the numerous quantitative mass spectrometry measurements required for identification and validation of novel intramembrane protease substrates for projects P1, P2 and P3. The results of the mass spec analyses will be required for all projects in the consortium, e.g. for the comparative bioinformatic analyses in P7. The platform has two high resolution Orbitrap mass spectrometers plus associated equipment, including nanoLCs, and has expertise with the required methods, such as label free quantification and different isotope label-containing methods. This includes 'stable isotope labelling with amino acids in cell culture' (SILAC) analyses in vitro and in vivo.