Proteomic platform

蛋白质组学平台将成为研究联盟的一个组成部分，并执行项目P1， P2和P3中鉴定和验证新型膜内蛋白酶底物所需的大量定量质谱测量。联盟中的所有项目都需要质谱分析的结果，例如P7中的比较生物信息学分析。该平台拥有两台高分辨率的Orbitrap质谱仪和相关设备，包括纳米lc，并具有所需方法的专业知识，例如无标签定量和不同的同位素含标签方法。这包括体外和体内的“细胞培养中氨基酸稳定同位素标记”（SILAC）分析。

The proteomic platform will be an integral part of the research consortium and performs the numerous quantitative mass spectrometry measurements required for identification and validation of novel intramembrane protease substrates for projects P1, P2 and P3. The results of the mass spec analyses will be required for all projects in the consortium, e.g. for the comparative bioinformatic analyses in P7. The platform has two high resolution Orbitrap mass spectrometers plus associated equipment, including nanoLCs, and has expertise with the required methods, such as label free quantification and different isotope label-containing methods. This includes 'stable isotope labelling with amino acids in cell culture' (SILAC) analyses in vitro and in vivo.