Polyploidy generates beauty: exploration of complex genomes of roses (Rosa L. sect. Caninae (DC.) Ser.)

尽管我们对多倍体基因组的理解取得了重大进展，但对多倍体基因组进化的许多方面仍然知之甚少。月季是一个重要的经济属，具有广泛的多倍性。然而，罗莎还有一个神秘之处，那就是拥有一种独特的生殖系统，称为永久奇数多倍体。在这里，我们建议使用细胞遗传学、分子和基因组方法来探索玫瑰有丝分裂和减数分裂。通过使用传统的rDNA和新开发的染色体特异性FISH探针，我们将跟踪亲本物种和自然杂交物种减数分裂过程中基因组的行为。对产生大量基因组序列的玫瑰基因组进行深度测序将有助于产生基因组和染色体特异性探针。我们期望这种方法将使我们能够获得关于构成染色体组的主要成分的信息，这些组分以不同的减数分裂行为为特征。

Despite significant progress in our understanding of polyploid genomes many aspects of their evolution remain poorly understood. Rosa represents an economically important genus with widespread polyploidy. However, Rosa is also enigmatic in having a unique reproduction system called 'permanent odd polyploidy'. Here we propose to explore Rosa mitosis and meiosis using cytogenetic, molecular and genomic approaches. By using conventional rDNA and newly developed chromosome-specific FISH probes, we are going to follow the behaviour of genomes during meiosis in parental species and natural hybrids. Deep sequencing of Rosa genomes yielding large amount of genomic sequences will be useful for generation of genome and chromosome-specific probes. We expect that such an approach will allow us to obtain information about the major components constituting the chromosome sets which are characterised by their different meiotic behaviour.