Serine ubiquitination and regulation of autophagy upon Legionella (E01 (C01))

丝氨酸泛素化和军团菌自噬调节 (E01 (C01))

• 批准号: 283341677
• 金额: --
• 依托单位: Institut für Physikalische und Theoretische Chemie
• 依托单位国家: 德国
• 项目类别: Collaborative Research Centres
• 财政年份: 2016
• 资助国家: 德国
• 起止时间: 2015-12-31 至 2022-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/283341677?language=en
• 关键词: Serine; ubiquitination; regulation; autophagy; upon

We will examine the impact of serine ubiquitination on the host autophagic pathway and how this might affect bacterial infection by Legionella. We will perform functional studies to elucidate the contribution of Legionella SidE proteins and RavZ in impairing host autophagy. We will use super-resolution microscopy to study the nanoscale protein organization around the Legionella-containing vacuole, focusing on autophagy, signaling proteins, and ubiquitination. We will develop a functional, spatiotemporal and molecular picture of processes that guard Legionella from autophagy-mediated clearance.

我们将研究丝氨酸泛素化对宿主自噬途径的影响，以及这可能如何影响军团菌的细菌感染。我们将进行功能研究，以阐明军团菌SidE蛋白和RavZ在损害宿主自噬中的贡献。我们将使用超分辨率显微镜来研究军团菌空泡周围的纳米级蛋白质组织，重点是自噬，信号蛋白和泛素化。我们将开发一个功能，时空和分子的过程，保护军团菌自噬介导的清除图片。