The role of phospholipase PlaF mediated membrane alterations for Pseudomonas aeruginosa virulence adaptations (A02#)

该项目旨在研究铜绿假单胞菌产生的胞内磷脂酶A如何发挥多种细胞反应，从而促进毒力适应。这种名为PlaF的酶改变了铜绿假单胞菌的内源性膜脂质组成，并通过尚不清楚的机制调节铜绿假单胞菌中的几种必需的和毒力相关的途径。在这里，我们将研究组装膜蛋白复合物的形成是依赖于PlaF：蛋白质的相互作用，并确定膜磷脂组合物的改变。在体外实验与重建的膜复合物将被用来阐明与先前确定的蛋白质和脂质效应器的相互作用的分子细节和它们的影响，调节生化功能的PLAF。预期的结果应该提供新的见解膜生物合成和毒力的脂质降解途径的功能。

The project addresses the question how an intracellular phospholipase A produced by the human pathogen Pseudomonas aeruginosa can exert a variety of cellular responses which contribute to virulence adap-tation. This enzyme named PlaF alters the endogenous membrane lipid composition and regulates several essential and virulence-related pathways in P. aeruginosa by still unknown mechanisms. Here, we will study the assembly of membrane protein complexes whose formation is dependent on PlaF:protein interactions and determine alterations of the membrane phospholipid composition. In vitro experiments with reconstituted membrane complexes will be used to elucidate molecular details of the interactions with the previously identified protein and lipid effectors and their effects for regulating the biochemical function of PlaF. The anticipated results should provide novel insights into the function of lipid degradation pathways for membrane biogenesis and virulence.