The role of phospholipase PlaF mediated membrane alterations for Pseudomonas aeruginosa virulence adaptations (A02#)

磷脂酶 PlaF 介导的膜改变对铜绿假单胞菌毒力适应的作用 (A02

• 批准号: 289530848
• 金额: --
• 依托单位: Institut für Molekulare Enzymtechnologie (IMET)
• 依托单位国家: 德国
• 项目类别: Collaborative Research Centres
• 财政年份: 2016
• 资助国家: 德国
• 起止时间: 2015-12-31 至 2022-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/289530848?language=en
• 关键词: role; phospholipase; PlaF; mediated; membrane

The project addresses the question how an intracellular phospholipase A produced by the human pathogen Pseudomonas aeruginosa can exert a variety of cellular responses which contribute to virulence adap-tation. This enzyme named PlaF alters the endogenous membrane lipid composition and regulates several essential and virulence-related pathways in P. aeruginosa by still unknown mechanisms. Here, we will study the assembly of membrane protein complexes whose formation is dependent on PlaF:protein interactions and determine alterations of the membrane phospholipid composition. In vitro experiments with reconstituted membrane complexes will be used to elucidate molecular details of the interactions with the previously identified protein and lipid effectors and their effects for regulating the biochemical function of PlaF. The anticipated results should provide novel insights into the function of lipid degradation pathways for membrane biogenesis and virulence.

该项目解决了由人类病原体铜绿假单胞菌产生的细胞内磷脂酶A如何发挥各种有助于毒力适应的细胞反应的问题。这种名为Plaf的酶改变了铜绿假单胞菌的内源性膜脂组成，并通过尚不清楚的机制调节了几个与毒力相关的必要途径。在这里，我们将研究膜蛋白复合体的组装，其形成依赖于Plaf：蛋白质相互作用，并确定膜磷脂组成的变化。重组膜复合体的体外实验将被用来阐明与先前发现的蛋白质和脂类效应物相互作用的分子细节，以及它们对调节Plaf生化功能的影响。预期的结果将为膜生物发生和毒力的脂类降解途径的功能提供新的见解。