Elucidation of the dynamics of the autophagosomal membrane-associated protein GABARAP by NMR spectroscopy (B03)

虽然自噬是细胞蛋白质降解的基本机制之一，但对自噬体膜隔间的调节仍不完全清楚。结合其他生物物理和生化技术，我们将利用最先进的核磁共振波谱技术来研究GABARAP的构象动力学和作用机制。GABARAP是一种关键的自噬相关蛋白，在自噬小体生物发生过程中，它在细胞质中以可溶性形式和以膜锚定形式交替调节膜系留和半融合。

Although autophagy is one of the fundamental cellular protein degradation mechanisms, the regulation of autophagosomal membrane compartments is still not fully understood. Using state-of-the-art NMR spectroscopy in concert with other biophysical and biochemical techniques we will investigate the conformational dynamics and functional mechanism of GABARAP, a key autophagy-related protein which alternates between a soluble form in the cytosol and a membrane-anchored form to mediate membrane tethering and hemifusion during autophagosome biogenesis.