Exploring the symbiotic interface of amoebal host and its nascent photosynthetic organelle in the amoeba Paulinella chromatophora (Rhizaria, Cercozoa) (B09)

我们的团队对细胞内细菌进化成遗传整合的细胞器感兴趣。在这个项目中，我们将集中在这个整合过程中，围绕不断发展的细胞器的包膜膜的作用。为此，我们利用一个独特的模型系统：阿米巴Paulinella chromatophora，其中包含新生的光合细胞器的蓝藻起源，称为色素。我们的主要目的将是确定的分子机制，使蛋白质易位跨双包膜膜封闭的色素细胞，并表征该膜系统的超微结构和脂质组成。

Our group is interested in the evolution of intracellular bacteria into genetically integrated cell organelles. In this project we will focus on the role of the envelope membranes that surround the evolving organelle during this integration process. For this purpose, we exploit a unique model system: the amoeba Paulinella chromatophora which contains nascent photosynthetic organelles of cyanobacterial origin, termed chromatophores. Our main aims will be to determine the molecular mechanisms that enable protein translocations across the double envelope membrane enclosing the chromatophore and to characterize ultra-structure and lipid composition of this membrane system.