Studies on Nanometer-sized Artificial Basement Membranes for Cell Compartmentalization in Three-dimensional Tissues

在今年，我们评估了通过阳离子-偶极相互作用构建的聚-L-赖氨酸/葡聚糖纳米膜（PLL/D NF）的屏障效应，以维持NHDF和HUVEC的区室化细胞共培养。PLL/D NF在细胞粘附、增殖和分化方面表现出与Col-IV/LM NF相当的能力。然而，值得注意的是，PLL/D NF维持区室化细胞共培养超过两周，比基于ECM蛋白的组装纳米膜长得多。此外，在融合的HUVEC单层下观察到IV型胶原的分泌，表明在体外培养的组织中天然BM的重建。PLL/D NF的有效屏障效应为体外构建有序的三维组织提供了有力的支持。

In this year, we evaluated the barrier effects of poly-L-lysine/dextran nanofilms (PLL/D NFs) constructed via the cation-dipole interaction to maintain compartmentalized cell co-culture of NHDF and HUVEC. PLL/D NFs exhibited comparable abilities to Col-IV/LM NFs in terms of cell adhesion, proliferation, and differentiation. However, it is worth noting that PLL/D NFs maintained compartmentalized cell co-culture for over two weeks, much longer than assembled nanofilms based on ECM proteins. Furthermore, the secretion of type IV collagen was observed under the confluent HUVEC monolayer, suggesting the re-construction of natural BMs in the in-vitro cultured tissues. The efficient barrier effects of PLL/D NFs provided a powerful support for constructing organized 3D tissues in vitro.