Studies on Nanometer-sized Artificial Basement Membranes for Cell Compartmentalization in Three-dimensional Tissues

纳米人工基底膜用于三维组织细胞区室化的研究

• 批准号: 21J12833
• 负责人: Zeng Jinfeng
• 金额: $ 0.96万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for JSPS Fellows
• 财政年份: 2021
• 资助国家: 日本
• 起止时间: 2021-04-28 至 2023-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-21J12833/
• 关键词: Artificial BMs; Compartmentalization; cation-dipole; surface charge; organized 3D tissue; ECM proteins; Cell migration; Tissue engineering

In this year, we evaluated the barrier effects of poly-L-lysine/dextran nanofilms (PLL/D NFs) constructed via the cation-dipole interaction to maintain compartmentalized cell co-culture of NHDF and HUVEC. PLL/D NFs exhibited comparable abilities to Col-IV/LM NFs in terms of cell adhesion, proliferation, and differentiation. However, it is worth noting that PLL/D NFs maintained compartmentalized cell co-culture for over two weeks, much longer than assembled nanofilms based on ECM proteins. Furthermore, the secretion of type IV collagen was observed under the confluent HUVEC monolayer, suggesting the re-construction of natural BMs in the in-vitro cultured tissues. The efficient barrier effects of PLL/D NFs provided a powerful support for constructing organized 3D tissues in vitro.

今年，我们评估了通过阳离子偶极相互作用构建的聚-L-赖氨酸/葡聚糖纳米膜（PLL/D NF）的屏障效应，以维持 NHDF 和 HUVEC 的区室化细胞共培养。 PLL/D NF 在细胞粘附、增殖和分化方面表现出与 Col-IV/LM NF 相当的能力。然而，值得注意的是，PLL/D NF 可以维持区室化细胞共培养两周以上，比基于 ECM 蛋白组装的纳米膜长得多。此外，在汇合的 HUVEC 单层下观察到 IV 型胶原蛋白的分泌，表明体外培养组织中天然 BM 的重建。 PLL/D NFs的高效屏障效应为体外构建有组织的3D组织提供了强有力的支持。

项目成果

Analysis of Thickness and Roughness Effects of Artificial Basement Membranes on Endothelial Cell Functions

人工基底膜厚度和粗糙度对内皮细胞功能的影响分析

• DOI: 10.2116/analsci.20scp10
• 发表时间: 2021
• 期刊: Analytical Sciences
• 影响因子: 1.6
• 作者: ZENG Jinfeng;MATSUSAKI Michiya
• 通讯作者: MATSUSAKI Michiya

Fabrication of Patterned Artificial Basement Membranes for Cell Compartmentalization in Organized 3D Tissues

用于组织 3D 组织中细胞区室化的图案化人工基底膜的制造

• 发表时间: 2021
• 作者: Yamaguchi Mina;Ishii Akihiro;Oikawa Itaru;Yamazaki Yusuke;Imura Masaaki;Takamura Hitoshi;Jinfeng Zeng and Michiya Matsusaki
• 通讯作者: Jinfeng Zeng and Michiya Matsusaki

Nanofilms Constructed by Cation-Dipole Interaction to Prevent Cell Migration for Cell Compartmentalization in 3D Tissues

通过阳离子-偶极相互作用构建的纳米膜可防止细胞迁移，从而实现 3D 组织中的细胞区室化

• 发表时间: 2022
• 作者: Tanaka Satoru;Ishii Akihiro;Yamaguchi Mina;Oikawa Itaru;Yamazaki Yusuke;Imura Masaaki;Takamura Hitoshi;樋口靖浩，布目佳央，高田仁志，工藤琢，早川晃弘，小林秀昭;Jinfeng Zeng and Michiya Matsusaki
• 通讯作者: Jinfeng Zeng and Michiya Matsusaki