窒素飢餓応答における植物特異的なDof転写因子の分子機能の解明

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In the first year, I have revealed that in model plant Arabidopsis, NIGT1 transcription factors, key regulators for N deficiency responses, directly repressed transcription of Dof1.7, and also revealed that knockout mutation of NIGT1 and Dof1.7 differentially affected the expression of three NRT2 genes encoding high-affinity nitrate transporters.In the second year, I examined the transcriptional relationship between Dof1.7 and the three NRT2 genes by ChIP and co-transfection assay and revealed that Dof1.7 directly activated the NRT2.5 promoter and indirectly activated the NRT2.4 promoter with the presence of NIGT1.1. I have completed a manuscript of this series of research and am preparing it for publication in a high-rank international journal.