窒素飢餓応答における植物特異的なDof転写因子の分子機能の解明

阐明植物特异性 Dof 转录因子在氮饥饿反应中的分子功能

• 批准号: 21J14077
• 负责人: 卓 梦娜
• 金额: $ 0.96万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for JSPS Fellows
• 财政年份: 2021
• 资助国家: 日本
• 起止时间: 2021-04-28 至 2023-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-21J14077/
• 关键词: 窒素欠乏; Dof転写因子

In the first year, I have revealed that in model plant Arabidopsis, NIGT1 transcription factors, key regulators for N deficiency responses, directly repressed transcription of Dof1.7, and also revealed that knockout mutation of NIGT1 and Dof1.7 differentially affected the expression of three NRT2 genes encoding high-affinity nitrate transporters.In the second year, I examined the transcriptional relationship between Dof1.7 and the three NRT2 genes by ChIP and co-transfection assay and revealed that Dof1.7 directly activated the NRT2.5 promoter and indirectly activated the NRT2.4 promoter with the presence of NIGT1.1. I have completed a manuscript of this series of research and am preparing it for publication in a high-rank international journal.

在第一年，我已经揭示了在模式植物拟南芥中，NIGT1转录因子，氮缺乏反应的关键调节因子，直接抑制Dof1.7的转录，并且还揭示了NIGT1和Dof1.7的敲除突变差异地影响编码高亲和力硝酸盐转运蛋白的三个NRT 2基因的表达。我通过ChIP和共转染实验检测了Dof1.7和三个NRT 2基因之间的转录关系，发现Dof1.7直接激活NRT 2.5启动子，并在NIGT1.1存在下间接激活NRT 2.4启动子。我已经完成了这一系列研究的手稿，并准备在一份高级国际期刊上发表。

项目成果

The Role of Dof1.7 Transcription Factor in NIGT1-Mediated Nitrogen Starvation Responses in Arabidopsis

Dof1.7 转录因子在 NIGT1 介导的拟南芥氮饥饿反应中的作用

• 发表时间: 2021
• 作者: Keita Takahashi;Hiroyuki Fujishiro;M D Ainslie;清水麻里奈;伊藤 靖浩;清水麻里奈;伊藤靖浩;卓 梦娜
• 通讯作者: 卓 梦娜

The role of Dof1.7 transcription factor in NIGT1-regulated nitrogen deficiency responses in Arabidopsis

Dof1.7转录因子在拟南芥NIGT1调节的氮缺乏反应中的作用

• 发表时间: 2022
• 作者: Ishii Akimitsu;Kamijyo Ryunosuke;Yamanaka Akinori;Yamamoto Akiyasu;卓 梦娜
• 通讯作者: 卓 梦娜

RWP-RK domain-containing transcription factors in the Viridiplantae: biology and phylogenetic relationships

Viridiplantae 中含有 RWP-RK 结构域的转录因子：生物学和系统发育关系

• DOI: 10.1093/jxb/erac229
• 发表时间: 2022
• 期刊: Journal of Experimental Botany
• 影响因子: 6.9
• 作者: Sakuraba Yasuhito;Zhuo Mengna;Yanagisawa Shuichi
• 通讯作者: Yanagisawa Shuichi

The Arabidopsis NLP7-HB52/54-VAR2 pathway modulates energy utilization in diverse light and nitrogen conditions

• DOI: 10.1016/j.cub.2022.10.024
• 发表时间: 2022-12-19
• 期刊: CURRENT BIOLOGY
• 影响因子: 9.2
• 作者: Ariga, Takuto;Sakuraba, Yasuhito;Yanagisawa, Shuichi
• 通讯作者: Yanagisawa, Shuichi