DNA modifications in the evolution of hematopoietic neoplasms (A01)

造血系统肿瘤进化中的DNA修饰（A01）

• 批准号: 290281113
• 金额: --
• 依托单位: Arbeitsgruppe Humanbiologie und Bioimaging
• 依托单位国家: 德国
• 项目类别: Collaborative Research Centres
• 财政年份: 2016
• 资助国家: 德国
• 起止时间: 2015-12-31 至 2019-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/290281113?language=en
• 关键词: DNA; modifications; evolution; hematopoietic; neoplasms

The aim of the project is to study the role and regulation of changes in DNA modifications and gene expression, using long-term in vitro cultured patient derived xenograft acute myeloid leukemia cells with and without selective pressure. We plan to look at genome-wide changes and at the conservation, variation and variance of patterns as a function of specific mutations in epigenetic modifiers (DNMT3A, TET2 and IDH1/2) and oncometabolite levels (2HG). The overall aim is to identify and counter epigenetic mechanisms that affect the response or resistance to therapy.

该项目的目的是研究DNA修饰和基因表达变化的作用和调节，使用长期体外培养的患者来源的异种移植急性髓性白血病细胞，有和没有选择压力。我们计划研究全基因组的变化，以及作为表观遗传修饰因子（DNMT 3A，TET 2和IDH 1/2）和癌代谢物水平（2 HG）中特定突变的功能的模式的保守性，变异性和方差。总体目标是识别和对抗影响对治疗的反应或抗性的表观遗传机制。