Fluorescence detection of hydrogen-bonding strength using ESIPT-type fluorescent probe

使用ESIPT型荧光探针进行氢键强度的荧光检测

• 批准号: 21K05121
• 负责人: 阿久津 和宏
• 金额: $ 2.66万
• 依托单位: 一般財団法人総合科学研究機構(総合科学研究センター(総合科学研究室)及び中性子科学センター(研究開発
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2021
• 资助国家: 日本
• 起止时间: 2021-04-01 至 2024-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-21K05121/
• 关键词: 水素結合; 蛍光プローブ; HBI; 中性子反射率; 界面

本研究は、化学的相互作用の一つである‘水素結合力’を定量的に分析するための新手法の開発を目的としており、我々は蛍光分子2-(2’-hydroxyphenyl)benzimidazole（HBI）を用いた簡易かつ高精度な蛍光分析法による水素結合力解析法の構築を目指している。令和4年度は、（1）蛍光-中性子反射率同時測定装置の開発、（2）（1）のための実験試料の作製とテスト実験、（3）量子科学計算によるHBI発光メカニズム解明を実施しており、それらの結果について具体的な内容を以下に示す。（1）蛍光-中性子反射率同時測定装置の開発：蛍光-中性子反射率同時測定用の試料ホルダーを作成し、蛍光スペクトル・中性子反射率どちらも問題なく測定できることを確認した。（2）（1）のための実験試料の作製とテスト実験：石英基板上にHBIを導入したセルロース薄膜を製膜し、蛍光スペクトル・中性子反射率を測定した。湿度100RH%の状態でテスト実験を実施し、蛍光スペクトル/中性子反射率の両方のデータで矛盾の無い結果が得られている。また、水素結合力をより精度よく分析するためのHBI分子の高度化を並行で進めており、高度化HBIの合成に成功している。（3）量子科学計算によるHBI発光メカニズム解明：HBIの発光プロセスに関与する化学種の最適化構造を検証し、TD-DFT法により、トルエン溶媒中におけるHBI誘導体の構造が最適化された。上記のように、装置の開発及びテスト実験は順調に進んでおり、一部の成果については国際会議等で報告している。令和5年度は、実試料を用いた蛍光-中性子反射率同時分析を実施するとともに、その成果を論文等で報告する。

In this study, chemical interactions were used to determine the binding force of water and water quantitatively. The purpose of this study was to analyze the binding force of water and water quantitatively. The purpose of this study was to determine the molecular weight of 2-(2'-hydroxyphenyl) benzimidazole (HBI). In this study, the interaction between water and water was analyzed quantitatively. In 2004, the photon-neutral reflectivity measurement device is open at the same time, (2) (1) the equipment is used to measure the light-neutral reflectivity at the same time, (2) (1) the equipment is used to measure the reflectivity at the same time, (2) (1) the equipment is used to measure the light-neutral reflectivity at the same time, (2) (1) the equipment for measuring the light-neutral reflectivity is open at the same time, (2) (1) the equipment for measuring the light-neutral reflectivity is open at the same time, (2) (1) the material is used for testing the reflectivity at the same time, (2) (1) the equipment for measuring the reflectivity of the light-neutral sub-reflectivity is open at the same time, (2) (1) the material is used for (1) the device for simultaneous measurement of light-neutral reflectivity is on: the simultaneous measurement of light-neutral reflectivity is based on the measurement of light-neutral reflectivity at the same time. The determination of the reflectivity of the neutral sub-reflectivity. (2) (1) the material is used to measure the reflectivity of the neutral substrates. (2) (1) the HBI on the quartz substrate is used to measure the reflectivity of the neutral substrates. The humidity of 100RH% is different from that of the neutral reflectivity. The results show that there is no error in the results. The combination of hydrogen and water was used to determine the accuracy of the analysis. The molecular height of HBI was analyzed and the synthesis of high-level HBI was successfully carried out. (3) Quantum science calculates the results of HBI photoluminescence analysis: HBI photoluminescence spectra and chemical agents are used to make the most effective chemical compounds, TD-DFT methods, and so on. In solvent media, the most effective chemical devices are made by the chemical agents of HBI. There are many reports such as the last report, the start-up of the equipment and the operation of the equipment, the review of the international conference and so on. At the same time, the reflectivity of the materials and materials in the year 5 are analyzed in the same time, such as the results of the reports.

项目成果

Current status of deuteration technologies in CROSS laboratory

CROSS实验室氘化技术现状

• 发表时间: 2023
• 作者: Yuki Ohno;Rei Tanaka;Yota Suzuki;Tomoaki Sugaya;Satoshi Iwatsuki;Masahiko Inamo;Koji Ishihara;Kazuhiro Akutsu
• 通讯作者: Kazuhiro Akutsu

Recent highlights and perspectives on chemical deuteration activities in CROSS

CROSS 化学氘化活动的最新亮点和观点

• 发表时间: 2023
• 作者: Kazuhiro Akutsu;Misaki Ueda;and Mitsuhiro Shibayama
• 通讯作者: and Mitsuhiro Shibayama

ANSTO-NDF(オーストラリア)

ANSTO-NDF(澳大利亚)

Recent Activity_Chemical Deuteration

近期活动_化学氘化

• 发表时间: 2021
• 作者: 鈴木翔大;田中玲衣;鈴木陽太;菅谷知明;石原浩二;Kazuhiro Akutsu
• 通讯作者: Kazuhiro Akutsu

New Design of a Sample Cell for Neutron Reflectometry in Liquid-Liquid Systems and Its Application for Studying Structures at Air-Liquid and Liquid-Liquid Interfaces

• DOI: 10.3390/app12031215
• 发表时间: 2022-02-01
• 期刊: APPLIED SCIENCES-BASEL
• 影响因子: 2.7
• 作者: Akutsu-Suyama, Kazuhiro;Yamada, Norifumi L.;Narita, Hirokazu
• 通讯作者: Narita, Hirokazu