植物根の局所栄養応答を地上部から制御する分子機構の解明

阐明控制地上植物根部局部营养反应的分子机制

• 批准号: 21K05324
• 负责人: 山崎 清志
• 金额: $ 2.66万
• 依托单位: The University of Tokyo (2022)Kyoto University (2021)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2021
• 资助国家: 日本
• 起止时间: 2021-04-01 至 2024-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-21K05324/
• 关键词: 栄養応答; 不均一栄養環境; 共発現ネットワーク; トランスクリプトーム; 屈性; 植物根

植物は 17 種類の無機栄養元素のうち 14 種を土壌から吸収している。この14種を標的として、【地上部→根】の栄養応答制御がみられる栄養元素の特定を試みた。根分けを併用した寒天栽培による実験系が困難であったため、昨年度に引き続き、根分けを併用した水耕栽培の実験系を改良した。水耕栽培の実験系のメリットとして、根を安定的に特定の水耕液にさらし続けることが容易である上に、ICP-MSによって水耕液の元素分析を行えば、根分けした植物根が各水耕液からどれだけの栄養を吸収したのか、数値化できる。つまり、水耕液の残存栄養濃度から植物が吸収した栄養量を見積もることができるが、もとの水耕液の栄養濃度が高すぎるため、植物が吸収した量が残存濃度に反映されない問題があった。昨年度の研究成果によりリンを標的栄養に絞り込んだので、様々なリン濃度を含む水耕液と根量の組み合わせ条件で栽培・ICP-MS測定をすることにより、根分け水耕開始後、1週間以内に地上部の栄養蓄積量と水耕液から低減した量に相関が得られることを確かめた。これらの成果から、どの実験条件でトランスクリプトーム解析用の根、地上部をサンプリングすればよいのか確定することができた。

There are 14 kinds of soil mineral absorption in plants and other non-organic elements. For each of the 14 types of equipment, the root system of the above ground should be used to control the specific test of the element of the system. The roots were cultivated in cold days, the roots were cultivated in cold days, the roots were introduced last year, and the roots were cultivated in water cultivation. For the cultivation of water-cultivated plants, the specific solution of water cultivation, the specific solution of root diazepam, the element analysis of water solution of ICP-MS, root, root and root. The absorption of water, the residual concentration of water, the absorption of plants, the absorption of water, the residual temperature of plant, the absorption of water, the residual temperature of plant, the absorption of water, the residual temperature of plant, the absorption of water, the residual temperature of plant, the absorption of water, the residual temperature of plant, the absorption of water, the residual temperature of plant, the absorption of water, the residual temperature of plant, the absorption of water, the residual temperature of plant, the absorption of water, the residual temperature of plant, the absorption of water, the residual temperature of plant, the absorption of water, the residual temperature of plant, the absorption of water, the residual temperature of plant, the absorption of water, the residual temperature of plant, the absorption of water, the residual temperature of plant, the absorption of water, the residual temperature of plant, the absorption of water, the residual temperature of plant, the absorption of water, the residual temperature of plant, the absorption of water In the last year, the results of the research were in good agreement with the results of the research in the field last year. The results of the research were as follows: the results of the research in the last year were as follows: last year, the results of the research were in good agreement with the results of the research in the year before and after the beginning of the irrigation, and within 1 year after the start of the water ploughing, and within 1 year after the start of the water ploughing, and within 1 year after the start of the water ploughing, and within 1 year after the start of the water ploughing, and within 1 year after the start of the water ploughing, the positive storage capacity of the upper part of the ground was higher than that of the water tillage solution. The results, conditions, conditions and conditions.