Application of body time detection by molecular timetable method for quality control of postharvest fruit and vegetables

新鲜农产品由于呼吸速率高，容易变质，因此极易变质。本研究研究了温度和控气贮藏对大豆芽中生物钟基因（GmCCA1、GmLHY、GmPRR7、GmGI、GmTOC1和GmLUX）表达和采后品质性状及其相关基因（GmFUM1、gmmcs、Gm2-OGDH、GmPPO1、GmPAL、GmDREB5）的影响。利用余弦曲线方程拟合qPCR法获得的基因表达量，成功发现大豆芽在恒定暗贮藏条件下存在昼夜节律。在对照（20℃-空气）豆芽中，clock基因表达有明显的节律性。相比之下，低温（10℃-空气）贮藏降低了GmLCL1、GmPRR7和GmTOC1的循环表达，也影响了GmGI和GmLUX的表达。此外，贮藏过程中的高浓度CO2（20℃-15% CO2 + 20% O2）通过影响每个基因的相位和振幅扰乱了生物钟；对于低氧浓度（20℃-5% O2），仅受振幅影响。有趣的是，低温、低氧和高CO2维持采后品质，包括减少呼吸、重量损失和褐变发生率。采后品质属性相关基因GmFUM1、GmCS、Gm2-OGDH、GmPPO1、GmPAL的表达行为也受到贮藏处理的影响。这一发现首次提出了生物钟紊乱与大豆芽采后质量维持之间具有生物学意义的联系。

Fresh produces are considered as highly perishable due to their high respiration rate and easy to deteriorate.In this research, the effect of temperature and controlled atmosphere storage on the expression of clock genes (GmCCA1, GmLHY, GmPRR7, GmGI, GmTOC1, and GmLUX) and postharvest quality characteristics and their related genes (GmFUM1, GmCS, Gm2-OGDH, GmPPO1, GmPAL, GmDREB5) in soybean sprouts were investigated. By fitting the gene expression level obtained using the qPCR method with the cosine curve equation, it was successfully found that the circadian rhythm existed under constant dark storage conditions of soybean sprouts. A significant rhythm in clock gene expression was observed in control (20 ℃-air) soybean sprouts. In contrast, low temperature (10 ℃-air) storage diminished the cyclic expression of GmLCL1, GmPRR7, and GmTOC1, which also affected GmGI and GmLUX expression. Additionally, high CO2 (20 ℃-15% CO2 + 20% O2) concentrations during storage disturbed the circadian clock by affecting the phase and amplitude of each gene; for low O2 (20 ℃-5% O2) concentrations, it was only affected by amplitude. Interestingly, low temperature, low O2, and high CO2 maintained postharvest quality, including reduced respiration, weight loss and browning incidence. The expression behaviors of postharvest quality attribute-related genes (GmFUM1, GmCS, Gm2-OGDH, GmPPO1, GmPAL) were also influenced by the storage treatments. The findings first suggest a biologically meaningful link between clock disruption and postharvest quality maintenance of soybean sprouts.