Application of body time detection by molecular timetable method for quality control of postharvest fruit and vegetables

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Fresh produces are considered as highly perishable due to their high respiration rate and easy to deteriorate.In this research, the effect of temperature and controlled atmosphere storage on the expression of clock genes (GmCCA1, GmLHY, GmPRR7, GmGI, GmTOC1, and GmLUX) and postharvest quality characteristics and their related genes (GmFUM1, GmCS, Gm2-OGDH, GmPPO1, GmPAL, GmDREB5) in soybean sprouts were investigated. By fitting the gene expression level obtained using the qPCR method with the cosine curve equation, it was successfully found that the circadian rhythm existed under constant dark storage conditions of soybean sprouts. A significant rhythm in clock gene expression was observed in control (20 ℃-air) soybean sprouts. In contrast, low temperature (10 ℃-air) storage diminished the cyclic expression of GmLCL1, GmPRR7, and GmTOC1, which also affected GmGI and GmLUX expression. Additionally, high CO2 (20 ℃-15% CO2 + 20% O2) concentrations during storage disturbed the circadian clock by affecting the phase and amplitude of each gene; for low O2 (20 ℃-5% O2) concentrations, it was only affected by amplitude. Interestingly, low temperature, low O2, and high CO2 maintained postharvest quality, including reduced respiration, weight loss and browning incidence. The expression behaviors of postharvest quality attribute-related genes (GmFUM1, GmCS, Gm2-OGDH, GmPPO1, GmPAL) were also influenced by the storage treatments. The findings first suggest a biologically meaningful link between clock disruption and postharvest quality maintenance of soybean sprouts.