Application of body time detection by molecular timetable method for quality control of postharvest fruit and vegetables

本研究以大豆芽菜为材料，研究了温度和气调贮藏对生物钟基因（GmCCA 1、GmLHY、GmPRR 7、GmGI、GmTOC 1和GmLUX）和采后品质及其相关基因（GmFUM 1、GmCS、Gm 2-OGDH、GmPPO 1、GmPAL和GmDREB 5）表达的影响。通过用余弦曲线方程拟合使用qPCR方法获得的基因表达水平，成功地发现在大豆芽的恒定黑暗储存条件下存在昼夜节律。在对照（20 ℃-空气）大豆芽中观察到时钟基因表达的显著节律。相反，低温（10 ℃-空气）储存减少了GmLCL 1，GmPRR 7和GmTOC 1的循环表达，这也影响了GmGI和GmLUX的表达。此外，高CO2浓度（20 ℃-15%CO2 + 20%O2）通过影响各基因的时相和振幅来扰乱生物钟，而低O2浓度（20 ℃-5%O2）仅影响振幅。有趣的是，低温，低O2，高CO2保持采后品质，包括减少呼吸，重量损失和布朗宁发病率。采后品质性状相关基因（GmFUM 1、GmCS、Gm 2-OGDH、GmPPO 1、GmPAL）的表达行为也受到贮藏处理的影响。这一发现首次提出了生物钟中断和豆芽采后质量保持之间的生物学意义的联系。

Fresh produces are considered as highly perishable due to their high respiration rate and easy to deteriorate.In this research, the effect of temperature and controlled atmosphere storage on the expression of clock genes (GmCCA1, GmLHY, GmPRR7, GmGI, GmTOC1, and GmLUX) and postharvest quality characteristics and their related genes (GmFUM1, GmCS, Gm2-OGDH, GmPPO1, GmPAL, GmDREB5) in soybean sprouts were investigated. By fitting the gene expression level obtained using the qPCR method with the cosine curve equation, it was successfully found that the circadian rhythm existed under constant dark storage conditions of soybean sprouts. A significant rhythm in clock gene expression was observed in control (20 ℃-air) soybean sprouts. In contrast, low temperature (10 ℃-air) storage diminished the cyclic expression of GmLCL1, GmPRR7, and GmTOC1, which also affected GmGI and GmLUX expression. Additionally, high CO2 (20 ℃-15% CO2 + 20% O2) concentrations during storage disturbed the circadian clock by affecting the phase and amplitude of each gene; for low O2 (20 ℃-5% O2) concentrations, it was only affected by amplitude. Interestingly, low temperature, low O2, and high CO2 maintained postharvest quality, including reduced respiration, weight loss and browning incidence. The expression behaviors of postharvest quality attribute-related genes (GmFUM1, GmCS, Gm2-OGDH, GmPPO1, GmPAL) were also influenced by the storage treatments. The findings first suggest a biologically meaningful link between clock disruption and postharvest quality maintenance of soybean sprouts.