ATP-dependent liquid phase separation during aging and neurodegeneration

项目正在按计划进行，没有出现重大问题。到目前为止所获得的数据如下：1)从最初的4个人IPSC系中，有3个成功地进行了培养、扩增和冷冻。2)分析了来自20、40和80个y/o人的IPSC的细胞增殖率和活力，发现克隆数显著减少。3)体外生物发光法测定了不同IPSC系的细胞内ATP水平，发现与20或40y/o的iPSC相比，80y/o的iPSCs的细胞内ATP水平显著降低。4)与年轻动物(8周)相比，老年动物的小鼠DRG原代培养中细胞内ATP水平的降低也得到证实，这支持我们在人类IPSC中的观察。

The project is going according to plan without significant problems. The data obtain so far are as followed:1) From the original 4 human iPSC lines, 3 have been successfully cultured, expanded, and cryopreserved.2) The cell proliferation rate and viability of iPSC from 20 y/o, 40 y/o and 80 y/o individuals have been analyzed and showed significant reduction in the number of colonies, number of cells and cell viability in older iPSCs compared to younger iPSCs.3) The intracellular levels of ATP in the various iPSC lines have been measured by in vitro bioluminescence assay and revealed a substantial reduction in ATP levels from 80 y/o iPSCs compared to 20 or 40 y/o iPSCs.4) Reduction in intracellular levels of ATP has also been confirmed in mouse DRG primary cultures from old animals (52 weeks) compared to younger animals (8 weeks), supporting our observations in human iPSCs.