ATP-dependent liquid phase separation during aging and neurodegeneration

工程正在按计划进行，没有出现重大问题。目前获得的数据如下：1)从最初的4个人类iPSC系中，成功培养、扩增和冷冻保存了3个。2)分析了20岁、40岁和80岁的iPSC的细胞增殖率和活力，结果表明，与年轻的iPSC相比，年龄较大的iPSC的菌落数量、细胞数量和细胞活力显著降低。3)通过体外生物发光法测定了不同iPSC细胞系的细胞内ATP水平，发现与20或40 y/o的iPSC相比，80 y/o的iPSC细胞内ATP水平显著降低。4)与年轻动物（8周）相比，老年动物（52周）的小鼠DRG原代培养物也证实了细胞内ATP水平的降低，这支持了我们在人类iPSCs中的观察结果。

The project is going according to plan without significant problems. The data obtain so far are as followed:1) From the original 4 human iPSC lines, 3 have been successfully cultured, expanded, and cryopreserved.2) The cell proliferation rate and viability of iPSC from 20 y/o, 40 y/o and 80 y/o individuals have been analyzed and showed significant reduction in the number of colonies, number of cells and cell viability in older iPSCs compared to younger iPSCs.3) The intracellular levels of ATP in the various iPSC lines have been measured by in vitro bioluminescence assay and revealed a substantial reduction in ATP levels from 80 y/o iPSCs compared to 20 or 40 y/o iPSCs.4) Reduction in intracellular levels of ATP has also been confirmed in mouse DRG primary cultures from old animals (52 weeks) compared to younger animals (8 weeks), supporting our observations in human iPSCs.