Investigation of alpha-synuclein associated synaptic dysfunction by unraveling the molecular mechanisms using 3D brain organoid model

首席研究员赵正俊博士因辞去OIST的职务，于2022年1月初决定中断“KAKENHI”项目，在10个月左右的时间里没有取得多少研究成果。根据第一年和第二年的特异性目标1，他成功地使用野生型和GBA1人类胚胎干细胞创建了hMLO-hSLO融合类器官，显示出相互的黑纹状体和纹状体-黑质突起。利用融合类器官，他证实了多巴胺能神经元和gaba能神经元在每个区域之间突触的形成。同时，他证实了-突触核蛋白聚集在融合类器官中的形成。

As a Principal Investigator, Dr. Junghyun Jo decided to discontinue the KAKENHI project in early January 2022 because he resigned from his position at OIST, there were not much research achievements for about 10 months. Following the Specific Aim 1 for Year 1 and 2, he has successfully created hMLO-hSLO fusion organoid using wildtype and GBA1 human embryonic stem cells that showed reciprocal nigro-striatal and striato-nigral projections. Using the fusion organoid, he has validated the formation of synapses between dopaminergic neurons and GABAergic neurons at each area. Also, he confirmed the formation of alpha-synuclein aggregation in the fusion organoids.