Development and application of an early detection method for postoperative recurrence of colorectal cancer using tears

泪液早期检测结直肠癌术后复发方法的开发与应用

• 批准号: 21K08778
• 负责人: 松田 武
• 金额: $ 2.66万
• 依托单位: Kobe University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2021
• 资助国家: 日本
• 起止时间: 2021-04-01 至 2024-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-21K08778/
• 关键词: 大腸癌; 術後サーベイランス; 肺転移; 肝転移; エクソソーム; microRNA; miRNA; 涙; 転移ニッチ; 骨髄由来抑制性細胞

本研究は、Liquid biopsy による原発性大腸癌術後再発の早期検出法の開発を目的とし、患者検体の採取および解析手段として涙液由来エクソソーム検出法 （TearExo 法) を用いる。本手法は①症例検体集積が簡便であり、迅速な前向き研究が実施可能、②エクソソームを確保するための抗体は選択可能で、病態・患者特異的に最適化したエクソソーム解析が可能、等の利点がある。転移臓器別抗体および患者個別抗体によるエクソソームを検出することで、個別検出を目指した次世代型精密医療の基盤構築を試みる。 まず、大腸癌細胞株を用いたエクソソーム検出と確認を行う。採取されたエクソソームをmiRNA検出で確認する。大腸癌細胞株由来や大腸癌原発巣切除例組織（オルガノイド）培養液 由来のエクソソームの単球系細胞への影響を検討する。この部分は超遠心とELISA 法で検証する。エクソソームと単球系細胞への影響を検討する。超遠心と ELISA 法で検証し、RNAシーケンスを行う。さらに、大腸癌原発巣切除例のエクソソーム検出と定量を行うために、術前後でTearExo 法の検証を行う。さらに、 同系が確立されれば、大腸癌同時性肝転移症例、同時性肺転移症例に対するエクソソーム検出とエクソソームの解析を行う。術前後でTearExo 法でのエクソソー ム検証を行う。さらに、原発性大腸癌切除例に対するエクソソーム検出例の集積と観察研究を行い、大規模前向き臨床研究へと進めたい。 本研究の成果は、大腸癌の再発の早期検出のみならず、臓器指向性転移の検出方法が期待され、臓器別のフォローを重点的に行うことにより、早期発見と早期 治療による治療性の向上を目指すものである。

In this study, Liquid biopsy was used to detect the primary cancer, and then the early screening method was used to determine the objective, and the analytical method was used to determine the origin of the solution. The TearExo method was used to determine the origin of the solution. In this maneuver, 1 case of the disease is characterized by constipation, rapid forward study, possibility, 2-month follow-up to make sure that the antibody is selected as possible, and the patient with disease is specialized. it is possible to analyze the possibility, and so on. To transfer the specific antibody to the patient, the specific antibody in the patient, the specific antibody, the antibody, the antibody. The cell lines of large and large cancer cells were confirmed by the use of cell lines. Please check out the confirmation button from miRNA. The origin of large breast cancer cell lines. The tissue culture solution for primary resection of large breast cancer is the origin of the culture medium for primary cancer resection. In some cases, the heart was surpassed and the ELISA method was used. I don't know. I don't know what to do. The ELISA method is very sensitive, and the RNA method is sensitive. In the case of primary resection of large cancer, quantitative examination was performed before and after surgery, and TearExo method was used before and after surgery. Pregnancy, homologous pregnancy, simultaneous liver metastases of major cancer, and simultaneous lung metastases were detected in all patients. Before and after the operation, the TearExo method was used to conduct a business operation. In the case of primary and primary resection of large cancer, there is a collection of cases of observation and study, and a large-scale forward bed study is in progress. In this study, we reported the results of this study, the early diagnosis of cancer, the method of directional transfer, the focus of the study, and the early treatment of breast cancer.