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Establishment of human pituitary adenoma cell-line and anti-vascular proliferation therapy for aggressive pituitary adenomas

人垂体腺瘤细胞系的建立及侵袭性垂体腺瘤的抗血管增殖治疗

基本信息

批准号：
21K09094
负责人：
阿久津博義
金额：
$ 2.66万
依托单位：
Dokkyo Medical University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2021
资助国家：
日本
起止时间：
2021-04-01 至 2024-03-31
项目状态：
已结题

项目摘要

筑波大学附属病院で手術された下垂体腺腫手術症例（非機能性・機能性下垂体PitNET、aggressive PitNET、pituitary carcinoma）において、手術摘出検体のうち病理診断の余剰分の腫瘍細胞を採取し培養した。摘出腫瘍細胞は、比較用に凍結して保存した。継代培養中には線維芽細胞の増殖が細胞株の増殖を阻害するため、研究分担者の有する技術であるE-cadherin coatingシャーレを使用し線維芽細胞の増生を抑制した上で継代培養し、腫瘍細胞のcolonial cloningを行った。すでに数種類のヒト下垂体腺腫細胞の数代の継代培養に成功し、培養細胞へ分泌刺激ホルモンを投与した際の培養液中のGH濃度を測定し、腫瘍細胞からのGH分泌能も確認できていたが、現在GH産生PitNET(densely granulated somatotroph tumor)とaggressive adenomaの一つであるsparsely granulated somatotroph tumorとpituitary carcinoma細胞株の樹立に成功した。GH産生PitNETではGH-RH刺激によりGH分泌応答があることも確認できている。ヒト下垂体腺腫の細胞株が樹立できればそれだけでも新規性があるため、論文作成にも着手している。今後はまず樹立株細胞が元々の摘出腫瘍細胞と一致していることを確認したのちに、免疫不全マウス皮下に腫瘍細胞の移植を行い、腫瘍形成を確認して動物モデルを樹立する予定である。

In the affiliated Hospital of the University of Tsukuba, there were cases of hand surgery (non-functional pituitary PitNET, aggressive PitNET, pituitary carcinoma), surgical extraction and pathological examination. Pick out the cell and save it with the box. In the process of cell culture, cell culture, cell growth, cell growth Several generations of prolapse gland cells have been successfully cultured, cultured for several generations, cultured to stimulate the secretion of the cells, to determine the degree of GH in the culture solution, to determine the secretion of GH in the culture solution, and to confirm the secretion of GH in the cells. Now the cell line of GH PitNET (densely granulated somatotroph tumor) aggressive adenomavirus sparsely granulated somatotroph tumor has been successfully established. GH gave birth to PitNET, GH-RH stimulation, GH secretion, response, confirmation. The vertical glands of the ovary and the cell lines of the prolapse gland were divided into two groups: the new standard, the new standard and the text. In the future, the cells were isolated from the plant, and the cells were consistent to confirm that they were infected, that the immune deficiency was subcutaneous, that the cells were transplanted, and that the animals were confirmed.