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おいしく食べるための唾液、その中枢神経機構の解明

唾液的美味及其中枢神经系统机制的阐明

基本信息

批准号：
21K11697
负责人：
美藤純弘
金额：
$ 2.58万
依托单位：
Okayama University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2021
资助国家：
日本
起止时间：
2021-04-01 至 2024-03-31
项目状态：
已结题

项目摘要

視床下部外側野ニューロンを電気刺激あるいは光刺激したときの唾液分泌を調べるために、電気刺激または光刺激のプローブを脳に刺入する位置を決める必要がある。そこで上唾液核に投射する視床下部外側野ニューロンの分布を検討するために、Wistar系ラット上唾液核にフルオロゴールド投与した。フルオロゴールドにより標識されたニューロンは視床下部外側野において吻尾側方向に広く分布していた（Bregma-1.8 mmから-4.5 mmの間）。また、コレラトキシンb Alexa Fluor 488をC57BL/6Jマウスの上唾液核に投与したときの視床下部外側野における分布を調べたところ、ラットの分布とよく似ていた。これらの結果から刺激プローブは吻尾側：Bregmaより -2.5 mm、外側：1.7 mm、深さ8.5 mmに刺入することを決めた。ラットの唾液分泌は非常に少ないので顎下腺導管にカニューレを挿入し、唾液の分泌圧を計測した。イソフルラン麻酔下で視床下部外側野に刺激電極を挿入し電気刺激したところ、唾液分泌はほとんど観察されなかった。舌を味覚刺激しても観察されなかった。ピロカルピンを腹腔内投与したところ、豊富な唾液分泌が観察された。以上の結果から麻酔薬の影響が考えらたので、その他の種々麻酔薬を試みたが唾液分泌は観察されなかった。一方、光刺激実験のため、視床下部外側野のオレキシン産生ニューロンに470 nm光で細胞を活性化させるチャネルロドプシン2を発現させることを試みているが、まだ成功していない。現在、自由行動下で視床下部外側野を電気刺激すること試みているが、この実験とともにチャネルロドプシン2の発現を試みる。

In the lower part of the bed, there was no electrical stimulation, no light stimulation, no saliva secretion, no light stimulation, no light stimulation, no electrical stimulation. The salivary nucleus was projected from the upper salivary nucleus in the lower part of the bed, the salivary nucleus was distributed in the lower part of the bed, the salivary nucleus was distributed in the lower part of the bed. In the lower part of the bed, the field is located in the direction of kissing the tail, and the distribution is located in the Bregma-1.8 mm room (between-4.5 mm). The saliva nuclei were injected into the saliva nucleus of b Alexa Fluor 488 C57BL/6J mice. The saliva nuclei were distributed in the lower part of the bed. The results of this study showed that the tail of Bregma was 2.5 mm, mm was 1.7, and the depth was 8.5 mm. The saliva secretion is very low. The inferior gland tube is very small. The saliva secretion is very low. In the lower part of the bed, the electric stimulation was activated in the lower part of the bed, and the saliva secretion was detected by saliva secretion. The taste of the tongue stimulates the pain. She was injected into the abdominal cavity and saliva secretion was detected by intraperitoneal injection. As a result of the above results, the results of the above results showed that the saliva secretion of all kinds of drugs and other kinds of drugs were detected. One side, light stimulation, light stimulation and light stimulation. Now, in the lower part of the free exercise, the electric stimulation in the lower part of the bed, the electric stimulation in the lower part of the bed, the electric stimulation in the lower part of the bed, the electric stimulation in the lower part of the bed, the electric stimulation in the lower part of the bed, the electric stimulation in the lower part of the bed, the electric stimulation in the lower part of the bed, the electric stimulation in the lower part of the bed, the electric stimulation in the lower part of the bed, the electric stimulation in the lower part of the bed, the electric stimulation in the lower part of the bed, the electric stimulation in the lower part of the bed, the electric stimulation in the lower part of the bed, the electric stimulation in the lower part of the bed, the electric stimulation in the lower part of the bed, the electric stimulation in the lower part of the bed, the electric stimulation in the lower part of the bed, the electric