The mechanical function of the nucleus during epithelial cell shape change and tissue deformation

我筛选了表达细胞骨架元件/调节因子和核成分突变等位基因的胚胎，用于核定位/背襞内陷缺陷。根据固定免疫染色胚胎的背褶表型，Shot（谱板）、Koi（太阳结构域蛋白）、Lamin、Suvar（3-3）和Rtn1（网状蛋白）与背褶内陷/核定位有关。免疫染色、活体成像和RT-PCR结果证明，极性蛋白Par1的定位和/或表达水平在核层破坏时可能受到干扰，该蛋白参与背襞内陷。我克隆了Klar的c端或KASH结构域，用于开发基于cry2 - cibn的光遗传学工具，该工具可以沿顶基细胞轴移动细胞核。

I screened embryos expressing mutant alleles of cytoskeletal elements/regulators and nuclear components for defects in nuclear positioning/dorsal fold invagination. Based on dorsal fold phenotypes in fixed, immunostained embryos, Shot (spectraplakin), Koi (Sun domain protein), Lamin, Suvar(3-3), and Rtn1 (reticulon) are implicated in dorsal fold invagination/nuclear positioning.The localization and/or expression levels of the polarity protein Par1, known to be involved in dorsal fold invagination, may be perturbed upon disruption of the nuclear lamina, as evidenced by immunostaining, live imaging, and RT-PCR results.I cloned the C-terminus of Klar or the KASH domain alone for the development of a CRY2-CIBN-based optogenetic tool that can move the nucleus along the apical-basal cell axis.