The mechanical function of the nucleus during epithelial cell shape change and tissue deformation

我筛选了表达细胞骨架元件/调节器和核成分突变等位基因的胚胎，以寻找核定位/背折内陷方面的缺陷。根据固定的、免疫染色的胚胎的背折叠表型，Sort(Spectraplakin)、Koi(太阳域蛋白)、Lamin、Suvar(3-3)和Rtn1(网状蛋白)与背折内陷/核定位有关。免疫染色、实时成像和RT-PCR结果表明，参与背折内陷的极性蛋白Par1的定位和/或表达水平可能会在核层破坏时受到干扰。我克隆了KlarC末端或Kash结构域，用于开发基于CRY2-CIBN的光遗传工具，可以沿着根尖细胞基轴移动细胞核。

I screened embryos expressing mutant alleles of cytoskeletal elements/regulators and nuclear components for defects in nuclear positioning/dorsal fold invagination. Based on dorsal fold phenotypes in fixed, immunostained embryos, Shot (spectraplakin), Koi (Sun domain protein), Lamin, Suvar(3-3), and Rtn1 (reticulon) are implicated in dorsal fold invagination/nuclear positioning.The localization and/or expression levels of the polarity protein Par1, known to be involved in dorsal fold invagination, may be perturbed upon disruption of the nuclear lamina, as evidenced by immunostaining, live imaging, and RT-PCR results.I cloned the C-terminus of Klar or the KASH domain alone for the development of a CRY2-CIBN-based optogenetic tool that can move the nucleus along the apical-basal cell axis.