Developing new therapeutic options for the treatment of liver cirrhosis through the epigenomic and transcriptome signature

我们正在分析不同的细胞通路在这两个过程中是如何变化的。在单核-ATAC-SEQ上，我们正在确认我已经用本体ATAC获得的一些结果。我们正在观察退化的肝星状细胞(HSC)如何比健康的HSC更容易获得染色质，尽管它们具有相似的基因表达谱，这是该项目的初始假设之一。然而，我们不仅在HSC中看到这种现象，而且在与HSC和肝细胞密切接触的肝窦内皮细胞中也发现了这种现象。

We were analyzing how the different cell pathways are changing during both processes. On single nuclei-ATAC-seq, we are confirming some results that I already obtainedwith bulk ATAC. We are observing how regressed Hepatic stellate cells (HSC) have more accessible chromatin than healthy HSC, although they havesimilar gene expression profiles, which was one of the initial hypothesis of this project. However, we are not seeing this only in HSC but alsoin Liver Sinusoidal Endothelial Cells, which are in close contact with HSC and hepatocytes.