iNOS遺伝子の発現を基軸とした喘息における末梢気道炎症の解明

基于 iNOS 基因表达阐明哮喘周围气道炎症

• 批准号: 20K17187
• 负责人: 西山 裕乃
• 金额: $ 2.41万
• 依托单位: Nagoya City University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Early-Career Scientists
• 财政年份: 2020
• 资助国家: 日本
• 起止时间: 2020-04-01 至 2024-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-20K17187/
• 关键词: 喘息; 遺伝子; CANO; 遺伝子研究; 治療抵抗性喘息; iNOS遺伝子; 遺伝子多型; Small Airway領域

喘息の病態には中枢気道のみならず、SA領域 の気道炎症が関与することが近年注目されており、ICSが到達しにくいSA領域の気道炎症の残存が難治化の一因と考えられている。呼気一酸化窒素濃度(FeNO)は気道のTh2炎症を表す非侵襲的なマーカーとして広く用いられており、その肺胞成分(CANO)はSA領域の気道炎症を反映し、喘息の重症度と相関することが示されている。FeNOは気道上皮細胞の誘導型NO合成酵素(iNOS)の発現亢進により産生が促進されるが、CANOの産生に関わる遺伝子制御は知られていない。最近、iNOS遺伝子のプロモータ領域の塩基配列のリピート数：(CCTTT)nとNOS2 mRNA発現が負の相関関係することが報告された。Short allele群(≦ 11)とlong allele群(>12)の2群間の解析では、NOS2 mRNA発現量に有意差を認めたものの、FeNO値は同等であった。既報でSA領域の気道炎症が喘息増悪に関与していることと、両群間で喘息の増悪回数に差異を認めることから「FeNOの差異としては現れないSA領域でのTh2炎症に相違がある」という仮説を立てた。当院に通院している喘息・慢性咳嗽患者、130人より血液を採取し、mRNA測定を行っている。mRNA採取が不十分であった21人に関しては2回目の血液採取を行った。症例登録を終了し、mRNA測定と結果の解析、喘息に寄与する遺伝子の測定・解析を進めている。

Wheezing disease, central disease, inflammation in the SA field, and inflammation in the field of SA. In recent years, attention has been paid to the disease, the disease in the SA field, the residual disease and the treatment of inflammation. Acylated asphyxia (FeNO), Th2 inflammation, non-invasive inflammation, lung cellular components (CANO), pulmonary cellular components (CANO), SA domain inflammation, wheezing severity, asthma severity, wheezing severity, wheezing severity, asthma severity, wheezing severity, asthma severity, FeNO epithelial cell-type NO synthesis enzyme (iNOS) has been found to be hyperactive, to promote growth, and to regulate the production of CANO, which is known to be sensitive. Recently, the number of reports in the field of iNOS is as follows: (CCTTT) n NOS2 mRNA. Short allele group (11), long allele group (& gt;12), NOS2 mRNA group, NOS2 mRNA group, and FeNO group. In both SA and SA fields, there is a significant difference in the number of responses between the two groups. There is a difference in the number of responses between the two groups. There is a difference in the number of responses between the two groups. There is no difference in the number of patients between the two groups. There is no difference in the number of patients between the two groups. There is no difference in the number of patients in the field of SA. There is a difference in the number of patients with Th2 inflammation. One hundred and thirty patients with wheezing chronic cough were admitted to the hospital, and their blood samples were collected and mRNA tests were performed. MRNA took blood samples from 21 people and looked back for blood samples. The symptoms were checked up, the results of mRNA test were analyzed, and the results of wheezing and analysis were improved.