光重合型架橋性ビニルエステルの分子量と多孔型吸収性骨補填材の骨形成率との関係

光聚合可交联乙烯基酯分子量与多孔可吸收骨移植材料成骨率的关系

• 批准号: 20K18602
• 负责人: 山田 知枝
• 金额: $ 2.75万
• 依托单位: Okayama University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Early-Career Scientists
• 财政年份: 2020
• 资助国家: 日本
• 起止时间: 2020-04-01 至 2024-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-20K18602/
• 关键词: 吸収性骨補填材; 骨形成タンパク徐放性

重合触媒濃度を3条件に設定して試料をラットの頭部に埋入し,埋入後1か月,2か月,4か月,9か月後にCT撮影を行い,試料埋入部を観察した.コラーゲンスポンジをφ8mm厚さ2mmにトリミングした.DVAの光重合触媒量は,カンファーキノン：第3級アミンをA群1.2：4.8w/v,B群0.6：2.4w/v,C群0.3：1.2w/vで調整し,BMP2は10μg/30μlDVAに調整した.DVA/コラーゲンスポンジ質量比は13/2とし,嫌気条件下で180秒間光照射し重合させた.8週齢のWistar系雄性ラット15匹を5匹ずつの3群に分け,麻酔下でA, B, C群の試料を頭蓋骨上正中に位置付けて骨膜と皮膚を縫合した.術後1, 2,4および9ヶ月後にX線μCT装置を用いて対象部位の観察を行った.その後,頭蓋骨ごと試料を採取し,試料の中央で前後に2等分して,前方部は脱灰後にパラフィン包埋して,ヘマトキシリン・エオジン(H-E)染色を行い,病理組織学的観察を行った.後方部はVillanueva骨染色後にMMA包埋して,非脱灰研磨標本を作製した.全組織量(TV),骨組織量(BV),PDVA量ならびに試料高さを計測し,BV/TVを算出した.μCTでは1か月後にはいずれの実験群でも骨組織の形成が見られたが,A群に比べるとB, C群の形成骨量は少なかったが,9か月後まで形態はほぼ維持された.病理組織学的には試料内に線維性組織が侵入して,既存骨表面から試料内に新生骨の部分的形成が認められた.PDVA表面には多核細胞が少数存在したが,炎症性細胞浸潤は認められなかった.BV/TVはA群ではB, C群に比べて有意に多い新生骨が見られた.また,A群の残存PDVA量はB, C群よりも有意に多く,試料高は C群よりも高かった.以上の結果から,光重合触媒量によってPDVAの吸収速度を調整可能であることが示唆された.

When the catalyst concentration is set under 3 conditions, the head of the sample is buried, and CT images are taken 1 month, 2 months, 4 months and 9 months after burial, and the embedded part of the sample is observed. DVA photo-catalyst concentration: φ8mm thickness: 2 mm. Group A: 1.2: 4.8w/v, Group B: 0.6: 2.4w/v, Group C: 0.3: 1.2 w/v,BMP2:10μg/30μ l DVA: 13/2, 180 s light exposure: 3 groups of 8-week Wistar male: 15 to 5 groups of 8-week Wistar male: 3 groups of 8:3 groups of 8-week Wistar male: 3 groups of 8. After 1, 2, 4, and 9 months of operation, X-ray μCT was used to observe the imaging site. After the skull was taken, the sample was divided into two parts in the middle and back, and the front part was deashed and embedded.(H-E) staining was carried out. Pathological observation was carried out. After Villanueva bone staining, MMA embedding was performed on the posterior part, and the non-deliming grinding process was performed on the posterior part. Total tissue volume (TV), bone volume (BV),PDVA volume and sample height were measured and BV/TV was calculated. The bone formation of group A was less than that of group B and group C after 1 month, and the morphology of group A was maintained after 9 months. Pathological histology showed that there were linear and dimensional tissue invasion in the sample, and the formation of new bone on the surface of the existing bone was recognized. There were A few multinucleated cells on the surface of PDVA, and inflammatory cell infiltration was recognized.BV/TV group A, B, C, and intentionally more new bone was observed. The residual PDVA quantity of group A is higher than that of group B and group C. As a result, it is possible to adjust the absorption rate of PDVA by adjusting the amount of photocatalyst.

项目成果

Newly designed flat surface artificial tongue system for speech improvement in glossectomy patient: A preliminary study

新设计的平面人工舌系统用于改善舌切除患者的言语能力：初步研究

• 发表时间: 2021
• 期刊: Journal of Prosthodontic Research
• 影响因子: 3.6
• 作者: Hori Kazuhiro;Uehara Fumiko;Yamaga Yoshio;Yoshimura Shogo;Okawa Jumpei;Tanimura Motoki;Ono Takahiro;Hiroaki Nagatsuka
• 通讯作者: Hiroaki Nagatsuka

Lack of occlusal support did not impact amyloid β deposition in APP knock-in mice

缺乏咬合支持不会影响 APP 敲入小鼠中的β淀粉样蛋白沉积

• 发表时间: 2021
• 期刊: Journal of Prosthodontic Research
• 影响因子: 3.6
• 作者: Murotani Yuki;Hatta Kodai;Takahashi Toshihito;Gondo Yasuyuki;Kamide Kei;Kabayama Mai;Masui Yukie;Ishizaki Tatsuro;Matsuda Ken-ichi;Mihara Yusuke;Fukutake Motoyoshi;Nishimura Yuichi;Akema Suzuna;Hagino Hiromasa;Higashi Kotaro;Togawa Hitomi;Maeda Yoshinobu;Asuka Murakami
• 通讯作者: Asuka Murakami